Citrus CAPS Marker Database

Locus name: Af1252

CAPS PCR info

PCR Anneal-Temp (℃) / Method: 62
Main product (bp): 600
Comment on PCR:
F-primer: CAAATGATGGTTAAGACGGAG
R-primer: TTCAAACCAGATCCTCGG

EST info

EST Accession number 1: CN189704
EST Accession number 2:

sequence-tagged site (STS seq)

STS:
CTTCCCATCTTAATACATCCTTCCAAGAAATTATATAACCTATTGCACATGAAATTTTTT
TGCACTTGAATCATGATGTACGGCATTCTGTTATGTTCCAGATTTCCTTAGCATACTGTG
CAATTGTGCGATCACTGCTGAATTTCCCACTGCCAGCTGTACTTAATATAGACATCTTCA
GCCACTTCTTCCGGTCCCTGACAGAAGACATACATCGCAATAACATAAGATACAATATTA
GAATTTATAAGAATATTGAATATCATAAAGACAGCCAGGATACAAGAACGAAACAGCAAC
TTGAGATCATTAGGGTTCACGTTTTATTTTAACTTACAGACTCAGTTCAGTTTGAGCTTA
CAGCTTATGTTGGCTAGTGAG
STS source: Okitsu 46
STS Forward / Reverse: Foward read
STS:
ATTCCCTGGAGGGGAACACTGGTTATGGTCGTGGTGATTATTTTCTAGTTGGTTATGACT
TCCCAAGTTACTTAGAGGCTCAGGACAGAGTTGACCAAGCTTACAAGTAAGACATTCGCA
ATCCTAATATCAAGTCCAAAAGTATTATATTTTGAAATAGACTTGTAATGCAGTATTTTA
TCTGCTTGCAAAAGAATCATATATAAAAACTTTACGAGACTGACTTTGATGACATCTGAC
CTTGTTGTCACTCTCTTTCCTCAAACACAAATGTGTGTCTTTTACCAGCCTAAACAAGGC
ACACTCTTCCTTAGATCTAAAATATCAAACTTACTCTTTCTTTACAAGAAAACCACACAC
CATGAAGTTGTTTTAACTTTCCCCTAGCTTGATACAAAAGGAAAATGTAGAGGTTGGACA
G
STS source: Okitsu 46
STS Forward / Reverse: Reverse read

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Experiments: CAPS pattern of Citrus collection

Experiments: Segregation pattern

Target on C. clementina (v1.0)

Transcript ID: Ciclev10027813m
Scaffold: scaffold_8
Start: 24202384
End: 24209439

Target on C. unshiu (v1.0)

AGI map info (Shimada et al. 2014)

KmMg map info (Omura et al. 2003)

JtTr map info (Omura et al. 2003)

Km/O41 map info (Nakano et al. 2003)

Rp/Sa map info (Ohta et al. 2011)

CAPS genotyping (Hybrid cv/Citrus collection)

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)