Locus name: Al0416
CAPS PCR info
PCR Anneal-Temp (℃) / Method: 58
Main product (bp): 1200
Comment on PCR: Multiple bands
F-primer: GACCACCACCGGACACTTGAT
R-primer: TTCTTCTTGGCTGCGGACTTG
EST info
EST Accession number 1: C95397
EST Accession number 2: DC892986
sequence-tagged site (STS seq)
STS:
TTACAGCAGCAAATCATCTGCTTCACACCAAGGGTGAAAGCAAGCAAAGCATGCTCTCGA GTCTGACCATCCTTGGAGATACCAGCTTCAAAACCACCGGTGGTGGAGTCAATGATAAGG ACGGCACAGTCAGCCTGTGAGGTACCGGTAATCATGTTCTTGATAAAGTCACGATGTCCG GGAGCATCAATGACGGTGCAGTAGTACTTGGTGGTCTCGAACTTCCACAAGGCAATATCA ATGGTAATACCACGTTCACGCTCAGCCTTGAGCTTGTCGAG |
STS source:
STS Forward / Reverse: Foward read
STS:
CAACCATGGGCTTGGTGGGAATCATCTTAACCATTCCAGCATCTCCATTCTTCAAGAACT TGGGCTCCTTCTCAAGCTCCTTGCCTGATCGCCTATCAATCTTCGTCACGAGCTCAGAAA ACTTGACGGCAATGTGGGAGGTGTGGCAGTCAAGCACTGGGGCATATCCATTTCCAATCT GGCCAGGGTGGTTCATGATGATGACCTGTGAGGTGAAGTTGGCAGCTTCCCTGGCCGGAT CATCCTTCGAGTTGGAAGCAACAAAACCACGCTTGAGATCCTTGACAGCAACGTTCTTCA |
STS source:
STS Forward / Reverse: Reverse read
Experiments: Restriction fragment profile
Compared cultivars (Left, (Middle), Right): Trovita orange/Kiyomi/Miyagawa-wase
Comment:
Figure legend:
Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:
Experiments: CAPS pattern of hybrid cultivar
Resriction enzyme: Msp1
Rank as CAPS marker: Others
Comment:
Figure legend:
CVName 1. Nagami, 2. Budha's, 3. Miyagawa, 4. Trovita, 5. Kiyomi, 6. Tachibana, 7. Koji, 8. Kinokuni (8' Hirakishu), 9. Mato pumello, 10. Kunip,
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)
Experiments: CAPS pattern of Citrus collection
Experiments: Segregation pattern
Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Msp1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: Jutaro unshu x Trovita orange
Restriction enzyme: Msp1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: Kiyomi x Okitsu41gou
Restriction enzyme: Msp1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Target on C. clementina (v1.0)
Transcript ID: Ciclev10028443m
Scaffold: scaffold_8
Start: 22316999
End: 22319482
Target on C. unshiu (v1.0)
Transcript ID: C_unshiu_00639:mRNA_12.1
Scaffold: C_unshiu_00639
Start: 90698
End: 99467
Gene function: Elongation factor 1-alpha
AGI map info (Shimada et al. 2014)
AGI LG: AGI-08
AGI cM: 23.023
KmMg map info (Omura et al. 2003)
KmMg LG:
KmMg cM:
JtTr map info (Omura et al. 2003)
JtTr LG: JtR-07
JtTr cM: 60.405
Km/O41 map info (Nakano et al. 2003)
Km/O41 LG: NAT-082
Km/O41 cM: 21.126
Rp/Sa map info (Ohta et al. 2011)
Rp/Sa LG:
Rp/Sa cM:
CAPS genotyping (Hybrid cv/Citrus collection)
CAPS genotyping:
Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)
Cultivar identification M/T/K:
SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)
SNP1:
DNA sequence around SNP1:
SNP2:
DNA sequence around SNP2:
SNP3:
DNA sequence around SNP3:
SNP4:
DNA sequence around SNP4: