Citrus CAPS Marker Database

Locus name: Al0602

CAPS PCR info

PCR Anneal-Temp (℃) / Method: 62
Main product (bp): 500
Comment on PCR:
F-primer: GACGGAAGCGGCGATGACT
R-primer: CCCGGAAAGCAAACAAGGAAA

EST info

EST Accession number 1: C95421
EST Accession number 2:

sequence-tagged site (STS seq)

STS:
NCATTTGCCAATCCACAGCCAAGTCATGAAGATNAAGAAAGAGTTTGAGAAGGTTAAGCA
TCCGTCGCTGCAGCAGCCTCGTAGATGAGAAGAGTGCTTCATGGTCTCAACAGGCAGCGC
CGCTCTCGTTCTCCGCTTGGCTTGCCTGATCATCATAGGCCTATTTCCGTTGCTGTTGAT
AGAAACACCTGCCGTGTCTGATCGCACACATGCTNTCTTGTTTTTCAAGATTTTAGTTCN
CACCAGTTTCTGTGTTCTTCTTGTTCTTACTTTTTTCTTCTTTTACCTCTGCCTNTCTTT
TTAATNTTTATATTTTGCTACGGCNGTACATATTCTTGNAGTAGTAGGATAAGGAGGAGG
AGACTTCTGGGATATTATTAAACCCCCTTCTC
STS source: Chuukanbohon NO 5
STS Forward / Reverse: Foward read
STS:
TATTGCCGTTGCTGTTGATAGAAACACCTGCNGTGTCTGATCGCACACATGCTTTCTTGT
TTTATACAAGATTTTAGTTCACACCAGTTTGCNGTGTTCTTCTTGTTCTTACTTTTTTCT
TCTTTTACCTCTGCCTTTCTTTTTAATTTTTATATTTTGCTACGGTTGTACATATTCTTG
GAGTAGTAGGATAAGGAGGAGGAGACTTCTGGGAG
STS source: Chuukanbohon NO 5
STS Forward / Reverse: Reverse read

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): Trovita orange/Kiyomi/Miyagawa-wase
Comment:
Figure legend:
Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:
Compared cultivars (Left, (Middle), Right): Frying dragon/Hyuuganatsu
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Resriction enzyme: Msp1
Rank as CAPS marker: Available marker
Comment: PCR_condition: 60C aneal
Figure legend:
CVName 1. Nagami, 2. Budha's, 3. Miyagawa, 4. Trovita, 5. Kiyomi, 6. Tachibana, 7. Koji, 8. Kinokuni (8' Hirakishu), 9. Mato pumello, 10. Kunip,
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)

Experiments: CAPS pattern of Citrus collection

Experiments: Segregation pattern

Population name: Kankitsu Chuukanbohon Nou8gou x Siamese Acidless
Restriction enzyme: Msp1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Msp1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: Jutaro unshu x Trovita orange
Restriction enzyme: Msp1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: Kiyomi x Miyagawa-wase
Restriction enzyme: Msp1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless

Target on C. clementina (v1.0)

Transcript ID: Ciclev10017248m
Scaffold: scaffold_2
Start: 27141148
End: 27141869

Target on C. unshiu (v1.0)

Transcript ID: C_unshiu_00017:mRNA_42.1
Scaffold: C_unshiu_00017
Start: 304994
End: 305523
Gene function: Hypothetical protein

AGI map info (Shimada et al. 2014)

AGI LG:
AGI cM:

KmMg map info (Omura et al. 2003)

KmMg LG: KmG-06
KmMg cM: 18.45

JtTr map info (Omura et al. 2003)

JtTr LG: JtR-06S
JtTr cM: 3.805

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG:
Km/O41 cM:

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG:
Rp/Sa cM:

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping: Hb

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K:

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1:
DNA sequence around SNP1:
SNP2:
DNA sequence around SNP2:
SNP3:
DNA sequence around SNP3:
SNP4:
DNA sequence around SNP4: