Citrus CAPS Marker Database

Locus name: Bf0027

CAPS PCR info

PCR Anneal-Temp (℃) / Method: 58
Main product (bp): 750
Comment on PCR:
F-primer: GCTGCACAGGCGTGGAA
R-primer: GCGGCTGCAAGTTCAACAATA

EST info

EST Accession number 1: DC885150
EST Accession number 2: DC885151

sequence-tagged site (STS seq)

STS:

AAATTCCTTTTGAGATACACGAACCCCCTTATTGGACAAACCTTGCGGTCTATGTGACAA
TTTCTAAAGACAAAATGGGTTCTACGTATCCCAAAAACAGGTATATGTGACCATTTCTGA
AAAAAAAGGGCGCACATAACCATTTTATCAAATCATAAGAGTGCTGCTGTCATTTATCCC
AGTTGGAATGATATAAATGTAAATTGGGAATCCTTTAGCAAGTCAAAGAACTACTCACCG
AGTAATCCCAGACAAGAGGATTGACAGCATTGGGAGTCTTCACCACTAAAAGGCTCTTGT
CCTTCTCCGATGGAAGAGGATTCACCGCATTAGCCACATC

STS source:
STS Forward / Reverse:

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:

Compared cultivars (Left, (Middle), Right): Frying dragon/Hyuuganatsu
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Experiments: CAPS pattern of Citrus collection

Experiments: Segregation pattern

Population name: Kankitsu Chuukanbohon Nou8gou x Siamese Acidless
Restriction enzyme: NC
Comment:
Figure legend:

A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless

Target on C. clementina (v1.0)

Transcript ID: Ciclev10032632m
Scaffold: scaffold_4
Start: 14565456
End: 14567860

Target on C. unshiu (v1.0)

Transcript ID: C_unshiu_00113:mRNA_23.1
Scaffold: C_unshiu_00113
Start: 169695
End: 172465
Gene function: Superoxide dismutase

AGI map info (Shimada et al. 2014)

AGI LG: AGI-07
AGI cM: 40.515

KmMg map info (Omura et al. 2003)

KmMg LG:
KmMg cM:

JtTr map info (Omura et al. 2003)

JtTr LG:
JtTr cM:

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG:
Km/O41 cM:

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG:
Rp/Sa cM:

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping:

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K:

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1: SI372
DNA sequence around SNP1:

GTATCCCAAAAACAGGTATATGTGACCATTTCTGAAAAAAAAGGGCGC[A/G]CATAACC
ATTTTATCAAATCATAAGAGTGCTGCTGTCATTTAT

SNP2:
DNA sequence around SNP2:

SNP3:
DNA sequence around SNP3:

SNP4:
DNA sequence around SNP4: