Citrus CAPS Marker Database

Locus name: Bf0115

CAPS PCR info

PCR Anneal-Temp (℃) / Method: 58
Main product (bp): 3000
Comment on PCR:
F-primer: TTAATGAGATACCGAAGTGTC
R-primer: GCATTGCCTATAAGAAGAATA

EST info

EST Accession number 1: DC885474
EST Accession number 2:

sequence-tagged site (STS seq)

STS:

CACATCTATTTTCCTTTCAATTTCCCTTTAAATCTCCTGGAGGGTTTATGATTTTGAATA
TCTCGTAAGGGCATAATCCCATTAATTGTTGGTTTTTCTTTTTCTAATTGGCATTTTTGA
AATTTTGCCCCTCAAGGACTTAAAATATGAGGAAAACTCTGACAAAGCAGTACAGTGCTT
GAATGATATGGTCACCAATGCTCTGATGCATGTGGAAGATTGTTTGAAGTACATGTCTGC
TTTAAGGGATCATGCTATATTCCGATTCTGTGCTATCCCTCAGGTGCGCTGCTTCTAGTA
TATTCTGATTTGAGAATTAATCTTGGAATTTTGGTATCAACATTTATTTTTAACTGTTAC
AATACCATCCACCCCAAATTAAACTTACCCCCCTCGAAGATTGGTGTGAGATGGATGAAT
CCATGACCTCTTGCTTCCACTGGAAGAGGCCAAACCAATT

STS source:
STS Forward / Reverse:

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:

Compared cultivars (Left, (Middle), Right): Frying dragon/Hyuuganatsu
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Experiments: CAPS pattern of Citrus collection

Rsetriction enzyme: NC
Comment:
Figure legend:

CV (No.1 Top) 101 Tahiti, 100 Rangpurlime, 102 Limonia, 104 LisbonL, 99 SweetLime, 92 Hirado, 87 MGF, 85 Kinukawa, 84 Hassaku, 83 Yamamikan, 78 Natsudaidai, 58 Rokugatu, Tr Trovita, 50 WNV, 44 Tankan, 42 Iyo,
(No.1 Bottom) 39 HgNt, 36 Ujukitsu, 32 Kawabata, 30 Shunkokan, 70 Yuzu, 54 Henka, 53 Ichang lemon, 37 Kunenbo, Mg Miyagawa, 31 Yatsushiro, 27 Kabuchii, 26 Ponkan, 25 Medet. 22. Dancy, 14 Tachibana, 11 Hirakishu (26' Ohta ponkan)

(No.2 Top) 6 Cleopatra, 4 CQ, 2 Fukuremikan, FD Hiryuu, 124 Largeleaf, 125 Small leaf, 141 CTV-S, 136 Rubidoaux,
(No.2 Bottom) FD, Hassaku, Km, F1, RP94, SA, 02, 04, 05, 06, 07, 08, 09, 10, 11, 13

BDZ BenDi Zao, SO Shuto, BP Banpeiyu

Experiments: Segregation pattern

Population name: Kiyomi x Okitsu41gou
Restriction enzyme: Hind3
Comment:
Figure legend:

A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless

Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Hind3
Comment:
Figure legend:

Population name: Kankitsu Chuukanbohon Nou8gou x Siamese Acidless
Restriction enzyme: Msp1
Comment:
Figure legend:

Target on C. clementina (v1.0)

Transcript ID: Ciclev10029066m
Scaffold: scaffold_8
Start: 6317205
End: 6322458

Target on C. unshiu (v1.0)

Transcript ID: C_unshiu_00484:mRNA_11.3
Scaffold: C_unshiu_00484
Start: 48216
End: 54250
Gene function: Squalene synthase

AGI map info (Shimada et al. 2014)

AGI LG: AGI-08
AGI cM: 58.587

KmMg map info (Omura et al. 2003)

KmMg LG:
KmMg cM:

JtTr map info (Omura et al. 2003)

JtTr LG:
JtTr cM:

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG: NAT-081
Km/O41 cM: 17.717

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG:
Rp/Sa cM:

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping:

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K:

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1: SI054
DNA sequence around SNP1:

GCTTTAAGGGATCATGCTATATTCCGATTCTGTGCTATCCCTCAGGTGCGCTGCTTCTAG
[T/C]ATATTCTGATTTGAGAATTAATCTTGGAATTTTGGTATCAACATTTATTTTTAAC
TGTTA

SNP2: SI295
DNA sequence around SNP2:

CTCGTAAGGGCATAATCCCATTAATTGTTGGTTTTTCTTTTTCTAATTGGCATTTTTGAA
[A/T]TTTTGCCCCTCAAGGACTTAAAATATGAGGAAAACTCTGACAAAGCAGTACAGTG
CTTGA

SNP3:
DNA sequence around SNP3:

SNP4:
DNA sequence around SNP4: