Citrus CAPS Marker Database

Locus name: Bf0145

CAPS PCR info

PCR Anneal-Temp (℃) / Method: 60
Main product (bp): 1600
Comment on PCR: Additional band (1200 bp)
F-primer: CATCTCCATCAGTCCCCACAG
R-primer: CAACCATCAAGGCAAGAACCA

EST info

EST Accession number 1: DC885769
EST Accession number 2:

sequence-tagged site (STS seq)

STS:
CCACGGCAGC AATGGACAAA ATGGAAGCAG CACTGCCTTG AATGCTGGAG TAACA
AATTT GGACAGTGAC AATGGGGCAG CAGGAAATAG TGGAGTTGGT GGCATAACTG
AAAGAATCAG TGGAAATGTG GTAGATGAAG TTTGGGTTGC TCAGAGAGAA GCTG
CCTTGA CTAAATTTCG CCAGAAAAGG AAAGAAAGGT GTTTCGGGAA GAGGGTAAC
T TTCTTTCTTT GCTTATGTTT TTACTTGGCT GCTAAGGATG GTTTATGTAG AGA
CTCTGTC CTGCTGGGTA GTTTTACTTG
STS source:
STS Forward / Reverse:
STS:
CAGTGACAATGGGGCAGCAGGAAATAGTGGAGTTGGTGGCATAACTGAAAGAATCAGTGG
AAATGTGGTAGATGAAGTTTGGGTTGCTCAGAGAGAAGCTGCCTTGACTAAATTTCG
STS source:
STS Forward / Reverse:

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:
Compared cultivars (Left, (Middle), Right): Frying dragon/Hyuuganatsu
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Experiments: CAPS pattern of Citrus collection

Rsetriction enzyme: Hae3
Comment:
Figure legend:
CV (No.1 Top) 101 Tahiti, 100 Rangpurlime, 102 Limonia, 104 LisbonL, 99 SweetLime, 92 Hirado, 87 MGF, 85 Kinukawa, 84 Hassaku, 83 Yamamikan, 78 Natsudaidai, 58 Rokugatu, Tr Trovita, 50 WNV, 44 Tankan, 42 Iyo,
(No.1 Bottom) 39 HgNt, 36 Ujukitsu, 32 Kawabata, 30 Shunkokan, 70 Yuzu, 54 Henka, 53 Ichang lemon, 37 Kunenbo, Mg Miyagawa, 31 Yatsushiro, 27 Kabuchii, 26 Ponkan, 25 Medet. 22. Dancy, 14 Tachibana, 11 Hirakishu (26' Ohta ponkan)
(No.2 Top) 6 Cleopatra, 4 CQ, 2 Fukuremikan, FD Hiryuu, 124 Largeleaf, 125 Small leaf, 141 CTV-S, 136 Rubidoaux,
(No.2 Bottom) FD, Hassaku, Km, F1, RP94, SA, 02, 04, 05, 06, 07, 08, 09, 10, 11, 13
BDZ BenDi Zao, SO Shuto, BP Banpeiyu
Rsetriction enzyme: Msp1
Comment:
Figure legend:
CV (No.1 Top) 101 Tahiti, 100 Rangpurlime, 102 Limonia, 104 LisbonL, 99 SweetLime, 92 Hirado, 87 MGF, 85 Kinukawa, 84 Hassaku, 83 Yamamikan, 78 Natsudaidai, 58 Rokugatu, Tr Trovita, 50 WNV, 44 Tankan, 42 Iyo,
(No.1 Bottom) 39 HgNt, 36 Ujukitsu, 32 Kawabata, 30 Shunkokan, 70 Yuzu, 54 Henka, 53 Ichang lemon, 37 Kunenbo, Mg Miyagawa, 31 Yatsushiro, 27 Kabuchii, 26 Ponkan, 25 Medet. 22. Dancy, 14 Tachibana, 11 Hirakishu (26' Ohta ponkan)
(No.2 Top) 6 Cleopatra, 4 CQ, 2 Fukuremikan, FD Hiryuu, 124 Largeleaf, 125 Small leaf, 141 CTV-S, 136 Rubidoaux,
(No.2 Bottom) FD, Hassaku, Km, F1, RP94, SA, 02, 04, 05, 06, 07, 08, 09, 10, 11, 13
BDZ BenDi Zao, SO Shuto, BP Banpeiyu

Experiments: Segregation pattern

Population name: Kankitsu Chuukanbohon Nou8gou x Siamese Acidless
Restriction enzyme: Hae3
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: Kiyomi x Okitsu41gou
Restriction enzyme: Msp1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Msp1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless

Target on C. clementina (v1.0)

Transcript ID: Ciclev10027909m
Scaffold: scaffold_8
Start: 22730839
End: 22737522

Target on C. unshiu (v1.0)

Transcript ID: C_unshiu_00158:mRNA_13.1
Scaffold: C_unshiu_00158
Start: 102548
End: 109253
Gene function: Pseudo-response regulator 37

AGI map info (Shimada et al. 2014)

AGI LG: AGI-08
AGI cM: 20.703

KmMg map info (Omura et al. 2003)

KmMg LG:
KmMg cM:

JtTr map info (Omura et al. 2003)

JtTr LG:
JtTr cM:

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG:
Km/O41 cM:

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG: RP-08
Rp/Sa cM: 5.775

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping:

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K: K

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1: SI272
DNA sequence around SNP1:
CAGTGACAATGGGGCAGCAGGAAATAGTGGAGTTGGTGGCATAACTGAAAGAATCAGTGG
[A/G]AATGTGGTAGATGAAGTTTGGGTTGCTCAGAGAGAAGCTGCCTTGACTAAATTTC
G
SNP2: SI327
DNA sequence around SNP2:
CAGTGACAATGGGGCAGCAGGAAATAGTGGAGTTGGTGGCATAACTGAAAGAATCAGTGG
[A/G]AATGTGGTAGATGAAGTTTGGGTTGCTCAGAGAGAAGCTGCCTTGACTAAATTTC
G
SNP3: SI368
DNA sequence around SNP3:
AGAAAAGGAAAGAAAGGTGTTTCGGGAAGAGGGTAACTTTCTTTCTTTGCTTATGTTTTT
[A/C]CTTGGCTGCTAAGGATGGTTTA
SNP4:
DNA sequence around SNP4: