Locus name: Bf0183
CAPS PCR info
PCR Anneal-Temp (℃) / Method: 58Main product (bp): 700
Comment on PCR:
F-primer: GGGCTGAAGGCTGAACAT
R-primer: TTTTCGGAATTACTGGGAAGA
EST info
EST Accession number 1: DC886132EST Accession number 2:
sequence-tagged site (STS seq)
STS:| GAATAATTAATACAAAGACTGCCGGCTTCATAGTTTGAGCTGATGGAGGAGAGAATTGTC ATCAACCGCCGTAGAAAATCCTTTGTTAAAACATTCACTTGCAAGAAAATCTCTGTAAAT AGGAGGATTTTCTTCAGATGTCAACTCTTTGATCGGCCCATAAGACTTTGGGATTTCTGC ATTATGTCCCATGAAAAAGCATGCCACGGAAACCCTTGGACCGACATGACCGGCGACTAC TCTGTGGTTAACACTCTTGAACTTGTCGTTTGATACAACCTGATTCAATTTTAAGACACC TCATTGTCGTGATGATTGATGAAGATTGGCATGATTGTGGAAAAAATATTCATTGTAATT GTGAAAGACAAACCTGGAGG |
STS Forward / Reverse:
Experiments: Restriction fragment profile
Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)Comment:
Figure legend:
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Experiments: CAPS pattern of hybrid cultivar
Experiments: CAPS pattern of Citrus collection
Rsetriction enzyme: NCComment:
Figure legend:
CV (No.1 Top) 101 Tahiti, 100 Rangpurlime, 102 Limonia, 104 LisbonL, 99 SweetLime, 92 Hirado, 87 MGF, 85 Kinukawa, 84 Hassaku, 83 Yamamikan, 78 Natsudaidai, 58 Rokugatu, Tr Trovita, 50 WNV, 44 Tankan, 42 Iyo,
(No.1 Bottom) 39 HgNt, 36 Ujukitsu, 32 Kawabata, 30 Shunkokan, 70 Yuzu, 54 Henka, 53 Ichang lemon, 37 Kunenbo, Mg Miyagawa, 31 Yatsushiro, 27 Kabuchii, 26 Ponkan, 25 Medet. 22. Dancy, 14 Tachibana, 11 Hirakishu (26' Ohta ponkan)
(No.1 Bottom) 39 HgNt, 36 Ujukitsu, 32 Kawabata, 30 Shunkokan, 70 Yuzu, 54 Henka, 53 Ichang lemon, 37 Kunenbo, Mg Miyagawa, 31 Yatsushiro, 27 Kabuchii, 26 Ponkan, 25 Medet. 22. Dancy, 14 Tachibana, 11 Hirakishu (26' Ohta ponkan)
(No.2 Top) 6 Cleopatra, 4 CQ, 2 Fukuremikan, FD Hiryuu, 124 Largeleaf, 125 Small leaf, 141 CTV-S, 136 Rubidoaux,
(No.2 Bottom) FD, Hassaku, Km, F1, RP94, SA, 02, 04, 05, 06, 07, 08, 09, 10, 11, 13
(No.2 Bottom) FD, Hassaku, Km, F1, RP94, SA, 02, 04, 05, 06, 07, 08, 09, 10, 11, 13
BDZ BenDi Zao, SO Shuto, BP Banpeiyu
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Experiments: Segregation pattern
Population name: Kankitsu Chuukanbohon Nou8gou x Siamese AcidlessRestriction enzyme: NC
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
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Target on C. clementina (v1.0)
Transcript ID: Ciclev10005208mScaffold: scaffold_9
Start: 5254416
End: 5256826
Target on C. unshiu (v1.0)
Transcript ID: C_unshiu_01106:mRNA_9.1Scaffold: C_unshiu_01106
Start: 77275
End: 79234
Gene function: Hypothetical protein
AGI map info (Shimada et al. 2014)
AGI LG:AGI cM:
KmMg map info (Omura et al. 2003)
KmMg LG:KmMg cM:
JtTr map info (Omura et al. 2003)
JtTr LG:JtTr cM:
Km/O41 map info (Nakano et al. 2003)
Km/O41 LG:Km/O41 cM:
Rp/Sa map info (Ohta et al. 2011)
Rp/Sa LG:Rp/Sa cM:
CAPS genotyping (Hybrid cv/Citrus collection)
CAPS genotyping:Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)
Cultivar identification M/T/K:SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)
SNP1: SI308DNA sequence around SNP1:
| GACTGCCGGCTTCATAGTTTGAGCTGATGGAGGAGAGAATTGTCATCAACCGCCGTAGAA [A/T]ATCCTTTGTTAAAACATTCACTTGCAAGAAAATCTCTGTAAATAGGAGGATTTTC TTCAG |
DNA sequence around SNP2:
DNA sequence around SNP3:
DNA sequence around SNP4: