Locus name: Cp0870
CAPS PCR info
PCR Anneal-Temp (℃) / Method: TdMain product (bp): 600
Comment on PCR:
F-primer: CCACCCAGTACACAGTTAACA
R-primer: GAGCAGCTTCTCCATTGTC
EST info
EST Accession number 1: DC892827EST Accession number 2:
sequence-tagged site (STS seq)
STS:| CATAGTCACCGGTAATGACTTCTCAACATTGTATGCTCCTCTCATCCGTGATGGTCGTAT GGAGAAATTCTACTGGGCACCTACAAGGGAGGACCGTATTGGTGTGTGCAAGGGTATCTT CAGGAATGACAATGTGGCTGATGACGACATTGTCAAGCTTGTTGACACCTTCCCCGGCCA ATCCATTGGTAAGTTTCCAGTTCCGTTGATGATGCAAACTCCCCATCAAATGCACACTAG CTAATCACTCATTTGACTGCGTTTTAGATTTCTTTGGAGCC |
STS Forward / Reverse:
Experiments: Restriction fragment profile
Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)Comment:
Figure legend:
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Experiments: CAPS pattern of hybrid cultivar
Resriction enzyme: Hinc2Rank as CAPS marker: no homozygous cultivar with minor allele
Comment:
Figure legend:
CVName 1. Nagami, 2. Budha's, 3. Miyagawa, 4. Trovita, 5. Kiyomi, 6. Tachibana, 7. Koji, 8. Kinokuni (8' Hirakishu), 9. Mato pumello, 10. Kunip,
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)
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Experiments: CAPS pattern of Citrus collection
Experiments: Segregation pattern
Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)Restriction enzyme: Hinc2
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
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Target on C. clementina (v1.0)
Transcript ID: Ciclev10031974mScaffold: scaffold_4
Start: 642157
End: 644823
Target on C. unshiu (v1.0)
Transcript ID: C_unshiu_00202:mRNA_16.4Scaffold: C_unshiu_00202
Start: 127322
End: 129988
Gene function: Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic
AGI map info (Shimada et al. 2014)
AGI LG: AGI-07AGI cM: 5.18
KmMg map info (Omura et al. 2003)
KmMg LG:KmMg cM:
JtTr map info (Omura et al. 2003)
JtTr LG:JtTr cM:
Km/O41 map info (Nakano et al. 2003)
Km/O41 LG:Km/O41 cM:
Rp/Sa map info (Ohta et al. 2011)
Rp/Sa LG:Rp/Sa cM:
CAPS genotyping (Hybrid cv/Citrus collection)
CAPS genotyping: HbCultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)
Cultivar identification M/T/K:SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)
SNP1: SI338DNA sequence around SNP1:
| TGTGTGCAAGGGTATCTTCAGGAATGACAATGTGGCTGATGACGACATTGTCAAGCTTGT [T/C]GACACCTTCCCCGGCCAATCCATTGGTAAGTTTCCAGTTCCGTTGATGATGCAAA CTCCC |
DNA sequence around SNP2:
DNA sequence around SNP3:
DNA sequence around SNP4: