Locus name: Cp0975
CAPS PCR info
PCR Anneal-Temp (℃) / Method: 54Main product (bp): 1600
Comment on PCR:
F-primer: TTCTTTCTCGAAGTCCCGTCT
R-primer: AACTGTTCCCGGTTGACATA
EST info
EST Accession number 1: CK936362EST Accession number 2:
sequence-tagged site (STS seq)
Experiments: Restriction fragment profile
Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)Comment:
Figure legend:
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Experiments: CAPS pattern of hybrid cultivar
Resriction enzyme: Hinf1Rank as CAPS marker: Disagree by Marco analysis
Comment: PCR_condition: 60C aneal
Figure legend:
CVName 1. Nagami, 2. Budha's, 3. Miyagawa, 4. Trovita, 5. Kiyomi, 6. Tachibana, 7. Koji, 8. Kinokuni (8' Hirakishu), 9. Mato pumello, 10. Kunip,
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)
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Experiments: CAPS pattern of Citrus collection
Experiments: Segregation pattern
Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)Restriction enzyme: Hinf1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
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Target on C. clementina (v1.0)
Transcript ID: Ciclev10012312mScaffold: scaffold_6
Start: 7924189
End: 7928192
Target on C. unshiu (v1.0)
Transcript ID: C_unshiu_00631:mRNA_15.1Scaffold: C_unshiu_00631
Start: 149702
End: 153660
Gene function: Isopentenyl pyrophosphate isomerase
AGI map info (Shimada et al. 2014)
AGI LG: AGI-07AGI cM: 29.152
KmMg map info (Omura et al. 2003)
KmMg LG:KmMg cM:
JtTr map info (Omura et al. 2003)
JtTr LG:JtTr cM:
Km/O41 map info (Nakano et al. 2003)
Km/O41 LG:Km/O41 cM:
Rp/Sa map info (Ohta et al. 2011)
Rp/Sa LG:Rp/Sa cM:
CAPS genotyping (Hybrid cv/Citrus collection)
CAPS genotyping:Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)
Cultivar identification M/T/K:SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)
SNP1:DNA sequence around SNP1:
DNA sequence around SNP2:
DNA sequence around SNP3:
DNA sequence around SNP4: