Citrus CAPS Marker Database

Locus name: Cp1738


CAPS PCR info

PCR Anneal-Temp (℃) / Method: Td
Main product (bp): 800
Comment on PCR:
F-primer: GCCTGGCAAATCTAATGG
R-primer: TCTGTTGTTAAACTCGCTGAC

EST info

EST Accession number 1: CK933293
EST Accession number 2:

sequence-tagged site (STS seq)

STS:
AACTCTCCATCTATCTCTTCTACGAGCCACAACTCACGACCCTTCCACGCCGCCCGATCC
GAAACGCCTGACCACGCTCCTCAGCTTCGGCCATAGCTCTCGGGCCACGGCCGCCGCCGT
CATCGAAGCCCTCATGGACCGCCTTCAGACAACCCACGATGCATCCGTCGCCATCAAGTC
CCTCATCGCCGTCCATCACATCGTCAAACATGGAAGCTTCATTCTCCAAGATCAGCTCTC
TGTTTATCCCTCCGCCGGTGGAAGAAACTATCTTAAATTGTCAAACTTCAGAGACAACAC
GACGCCGCTTACTTGGGAACTCTCTTCGTGGGTCAGATGGTACGCTTTATATCTCGAACA
CCTCTTGTCCACTTCTAGGGTTTTGGGTTTCTTCCTT
STS source:
STS Forward / Reverse:

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Resriction enzyme: Rsa1
Rank as CAPS marker: Available marker
Comment:
Figure legend:
CVName 1. Nagami, 2. Budha's, 3. Miyagawa, 4. Trovita, 5. Kiyomi, 6. Tachibana, 7. Koji, 8. Kinokuni (8' Hirakishu), 9. Mato pumello, 10. Kunip,
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)

Experiments: CAPS pattern of Citrus collection


Experiments: Segregation pattern

Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Rsa1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless

Target on C. clementina (v1.0)

Transcript ID: Ciclev10025941m
Scaffold: scaffold_7
Start: 5388458
End: 5389981

Target on C. unshiu (v1.0)

Transcript ID: C_unshiu_00303:mRNA_12.1
Scaffold: C_unshiu_00303
Start: 100918
End: 102276
Gene function: Putative clathrin assembly protein

AGI map info (Shimada et al. 2014)

AGI LG:
AGI cM:

KmMg map info (Omura et al. 2003)

KmMg LG:
KmMg cM:

JtTr map info (Omura et al. 2003)

JtTr LG:
JtTr cM:

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG:
Km/O41 cM:

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG:
Rp/Sa cM:

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping: Hb

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K:

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1: SI237
DNA sequence around SNP1:
AAACTTCAGAGACAACACGACGCCGCTTACTTGGGAACTCTCTTCGTGGGTCAGATGGTA
[T/C]GCTTTATATCTCGAACACCTCTTGTCCACTTCTAGGGTTTTGGGTTTCTTCCTT
SNP2: SI296
DNA sequence around SNP2:
GGAAGCTTCATTCTCCAAGATCAGCTCTCTGTTTATCCCTCCGC[T/C]GGTGGAAGAAA
CTATCTTAAATTGTCAAACTTCAGAGACAACACGACGCCGCTTACTTGG
SNP3:
DNA sequence around SNP3:
SNP4:
DNA sequence around SNP4: