Locus name: Cp2190
CAPS PCR info
PCR Anneal-Temp (℃) / Method: TdMain product (bp): 600
Comment on PCR:
F-primer: GGAAATTACTGCCACGACGTT
R-primer: AACAAAGCCTCCTCCGAAGA
EST info
EST Accession number 1: DC889480EST Accession number 2:
sequence-tagged site (STS seq)
STS:| TCATTTCCAACGTCCTCAATGGCTTGTCCATTTCTTGCAGCCTGATTGCTAAAGTGGGTC ATGATTTTAAATACGAAACTAGTTATCGTCCAATTATCGTGCCCACTTCTAAAACGACAG TTTTTCACGCGTATTTTGATTCGATTTCGGCTCCGAGTGGGCACTCAGATCGGGTCCTGA AACGGGTTTGCTCGTGTGAACCGATTACACCGTCGGATCTTCCAGATGGGAAGTTTGAT |
STS Forward / Reverse:
Experiments: Restriction fragment profile
Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)Comment: PCR_condition: 60C aneal
Figure legend:
 |
Experiments: CAPS pattern of hybrid cultivar
Resriction enzyme: Rsa1Rank as CAPS marker: no homozygous cultivar with minor allele
Comment:
Figure legend:
CVName 1. Nagami, 2. Budha's, 3. Miyagawa, 4. Trovita, 5. Kiyomi, 6. Tachibana, 7. Koji, 8. Kinokuni (8' Hirakishu), 9. Mato pumello, 10. Kunip,
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)
 |
Experiments: CAPS pattern of Citrus collection
Experiments: Segregation pattern
Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)Restriction enzyme: Rsa1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
 |
Target on C. clementina (v1.0)
Transcript ID: Ciclev10028681mScaffold: scaffold_8
Start: 24416582
End: 24418982
Target on C. unshiu (v1.0)
Transcript ID: C_unshiu_00021:mRNA_82.1Scaffold: C_unshiu_00021
Start: 526890
End: 529253
Gene function: Kinase PfkB
AGI map info (Shimada et al. 2014)
AGI LG: AGI-08AGI cM: 13.78
KmMg map info (Omura et al. 2003)
KmMg LG:KmMg cM:
JtTr map info (Omura et al. 2003)
JtTr LG:JtTr cM:
Km/O41 map info (Nakano et al. 2003)
Km/O41 LG:Km/O41 cM:
Rp/Sa map info (Ohta et al. 2011)
Rp/Sa LG:Rp/Sa cM:
CAPS genotyping (Hybrid cv/Citrus collection)
CAPS genotyping: HbCultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)
Cultivar identification M/T/K:SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)
SNP1:DNA sequence around SNP1:
DNA sequence around SNP2:
DNA sequence around SNP3:
DNA sequence around SNP4: