Citrus CAPS Marker Database

Locus name: Fb0110

CAPS PCR info

PCR Anneal-Temp (℃) / Method: 60
Main product (bp): 2400
Comment on PCR:
F-primer: CACGAACTGTGCTTGTCA
R-primer: CCTTAAAATTTGCCTCCTAAT

EST info

EST Accession number 1: DC888023
EST Accession number 2: DC888024

sequence-tagged site (STS seq)

STS:
TGTGTATTTATGTGCAAATAAAAGTACTCAGTGTGATGTTGATTTCATTTTTACTTGCTT
TTGCAGAGAGAAAGATGCTGACACAAGGGGCTTTCTCGAAGCAGCGAAAGCATGTCTTCA
AATTTGTTGCGTCCATGGAGTACCTCTGCTGATTAATGACCGCATCGATATTGCCCTTGC
TTGTGATGCTGATGGTGTGCATCTAGGTCAGTCTGACATGCCTGCTCGTACTGCCCGTGC
TCTTCTTGGTCCTGATAAGATCATTGGCGTATCGTGCAAAACACCTGAAGAAGCCCATCA
AGCTTGGATTGATGGTGCCAATTACATTGGTTGTGGTGGAGTATATCGAACTAACACAAA
GGCAAATAATCTCACTGTCGGTTTGGATGGATTGAAAACTGTTTGTTTGGCTTCCAAGTT
ACCTGTGGTTGCAATTGGTGGCATCGGCATTTCAAATGCATCTGACGTGATGAAAATTGG
TGTATCAAATCTTAAAGGTGTTGCCGTTGTGTCAGCTCTTTTTGATAGGGAATGTATCTT
G
STS source:
STS Forward / Reverse:

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:
Compared cultivars (Left, (Middle), Right): Frying dragon/Hyuuganatsu
Comment:
Figure legend:
Compared cultivars (Left, (Middle), Right): Trovita orange/Kiyomi/Miyagawa-wase
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Experiments: CAPS pattern of Citrus collection

Rsetriction enzyme: NC
Comment:
Figure legend:
CV (No.1 Top) 101 Tahiti, 100 Rangpurlime, 102 Limonia, 104 LisbonL, 99 SweetLime, 92 Hirado, 87 MGF, 85 Kinukawa, 84 Hassaku, 83 Yamamikan, 78 Natsudaidai, 58 Rokugatu, Tr Trovita, 50 WNV, 44 Tankan, 42 Iyo,
(No.1 Bottom) 39 HgNt, 36 Ujukitsu, 32 Kawabata, 30 Shunkokan, 70 Yuzu, 54 Henka, 53 Ichang lemon, 37 Kunenbo, Mg Miyagawa, 31 Yatsushiro, 27 Kabuchii, 26 Ponkan, 25 Medet. 22. Dancy, 14 Tachibana, 11 Hirakishu (26' Ohta ponkan)
(No.2 Top) 6 Cleopatra, 4 CQ, 2 Fukuremikan, FD Hiryuu, 124 Largeleaf, 125 Small leaf, 141 CTV-S, 136 Rubidoaux,
(No.2 Bottom) FD, Hassaku, Km, F1, RP94, SA, 02, 04, 05, 06, 07, 08, 09, 10, 11, 13
BDZ BenDi Zao, SO Shuto, BP Banpeiyu

Experiments: Segregation pattern

Population name: Kankitsu Chuukanbohon Nou8gou x Siamese Acidless
Restriction enzyme: Hinc2
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Hinf1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: Jutaro unshu x Trovita orange
Restriction enzyme: Hinf1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: Kiyomi x Miyagawa-wase
Restriction enzyme: Hinf1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless

Target on C. clementina (v1.0)

Transcript ID: Ciclev10000900m
Scaffold: scaffold_5
Start: 8512380
End: 8517835

Target on C. unshiu (v1.0)

Transcript ID: C_unshiu_00033:mRNA_4.1
Scaffold: C_unshiu_00033
Start: 37755
End: 43368
Gene function: Bifunctional protein thiED

AGI map info (Shimada et al. 2014)

AGI LG: AGI-09
AGI cM: 85.81

KmMg map info (Omura et al. 2003)

KmMg LG: KmG-09
KmMg cM: 23.401

JtTr map info (Omura et al. 2003)

JtTr LG: JtR-09S
JtTr cM: 5.362

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG:
Km/O41 cM:

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG:
Rp/Sa cM:

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping:

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K:

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1: SI246
DNA sequence around SNP1:
GAAAGATGCTGACACAAGGGGCTTTCTCGAAGCAGCGAAAGCATGTCTTCAAATTTGTTG
[T/C]GTCCATGGAGTACCTCTGCTGATTAATGACCGCATCGATATTGCCCT
SNP2:
DNA sequence around SNP2:
SNP3:
DNA sequence around SNP3:
SNP4:
DNA sequence around SNP4: