Citrus CAPS Marker Database

Locus name: Fb0223


CAPS PCR info

PCR Anneal-Temp (℃) / Method: 58
Main product (bp): 1200
Comment on PCR:
F-primer: CTCCGCTTTAGTTTGTGG
R-primer: TTTCCGATAGATTTCCTCTTC

EST info

EST Accession number 1: DC888148
EST Accession number 2: DC888149

sequence-tagged site (STS seq)

STS:
TCATTTTAAATTTCATTTTCTCTGTTGTTATTGAGACAAAAATGTAGGAGGATTTGGAGA
ACATATGCATAATTCAGGGAGGAGCAGTGTCGTAGTTACAGAACAAATTCGAGGCCAATT
AATGCCGGAGGAATTGGATGTGCCGCGCATTGCGAATCAATGCCGGAGGAAATGGGATTC
ATTGCTTGATGAGTACAAAAAGATAATTGTGCGATCAAGGACTTTTCCTAATTCCCAAAC
TCAAACTCATACTGATTTCTTTCCACCCAATTTTGACCGTGAGCACTTTAAAGCCATTCA
TGATTTCGTTATGTCTAAGGATAACCGATTTGATGATACTGACCCAGATTCAGATACCGA
TCCTGAAGCTGACTTCTCTGAAGCAATTTCACAAGCACAACTAGGTAACTTTTAACTTCT
CTCTCTTGTCTTGGATTGGGAGGACTTCTCATCCACCTCTATAATAAGAACATACACACA
TACTCTTTATTTTTATCTCCA
STS source:
STS Forward / Reverse:

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar


Experiments: CAPS pattern of Citrus collection


Experiments: Segregation pattern

Population name: Kankitsu Chuukanbohon Nou8gou x Siamese Acidless
Restriction enzyme: NC
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless

Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Nde2
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless

Target on C. clementina (v1.0)

Transcript ID:
Scaffold:
Start:
End:

Target on C. unshiu (v1.0)

Transcript ID: C_unshiu_00303:mRNA_13.1
Scaffold: C_unshiu_00303
Start: 108935
End: 110191
Gene function: E3 ubiquitin-protein ligase ATL6

AGI map info (Shimada et al. 2014)

AGI LG:
AGI cM:

KmMg map info (Omura et al. 2003)

KmMg LG:
KmMg cM:

JtTr map info (Omura et al. 2003)

JtTr LG:
JtTr cM:

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG:
Km/O41 cM:

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG: RP-08
Rp/Sa cM: 15.967

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping:

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K:

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1: SI240
DNA sequence around SNP1:
TGTTGTTATTGAGACAAAAATGTAGGAGGATTTGGAGAACATATGCATAATTCAGGGAGG
[A/G]GCAGTGTCGTAGTTACAGAACAAATTCGAGGCCAATTAAT
SNP2:
DNA sequence around SNP2:
SNP3:
DNA sequence around SNP3:
SNP4:
DNA sequence around SNP4: