Locus name: Fb0223
CAPS PCR info
PCR Anneal-Temp (℃) / Method: 58
Main product (bp): 1200
Comment on PCR:
F-primer: CTCCGCTTTAGTTTGTGG
R-primer: TTTCCGATAGATTTCCTCTTC
EST info
EST Accession number 1: DC888148
EST Accession number 2: DC888149
sequence-tagged site (STS seq)
STS:
TCATTTTAAATTTCATTTTCTCTGTTGTTATTGAGACAAAAATGTAGGAGGATTTGGAGA ACATATGCATAATTCAGGGAGGAGCAGTGTCGTAGTTACAGAACAAATTCGAGGCCAATT AATGCCGGAGGAATTGGATGTGCCGCGCATTGCGAATCAATGCCGGAGGAAATGGGATTC ATTGCTTGATGAGTACAAAAAGATAATTGTGCGATCAAGGACTTTTCCTAATTCCCAAAC TCAAACTCATACTGATTTCTTTCCACCCAATTTTGACCGTGAGCACTTTAAAGCCATTCA TGATTTCGTTATGTCTAAGGATAACCGATTTGATGATACTGACCCAGATTCAGATACCGA TCCTGAAGCTGACTTCTCTGAAGCAATTTCACAAGCACAACTAGGTAACTTTTAACTTCT CTCTCTTGTCTTGGATTGGGAGGACTTCTCATCCACCTCTATAATAAGAACATACACACA TACTCTTTATTTTTATCTCCA |
STS source:
STS Forward / Reverse:
Experiments: Restriction fragment profile
Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:
Experiments: CAPS pattern of hybrid cultivar
Experiments: CAPS pattern of Citrus collection
Experiments: Segregation pattern
Population name: Kankitsu Chuukanbohon Nou8gou x Siamese Acidless
Restriction enzyme: NC
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Nde2
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Target on C. clementina (v1.0)
Transcript ID:
Scaffold:
Start:
End:
Target on C. unshiu (v1.0)
Transcript ID: C_unshiu_00303:mRNA_13.1
Scaffold: C_unshiu_00303
Start: 108935
End: 110191
Gene function: E3 ubiquitin-protein ligase ATL6
AGI map info (Shimada et al. 2014)
AGI LG:
AGI cM:
KmMg map info (Omura et al. 2003)
KmMg LG:
KmMg cM:
JtTr map info (Omura et al. 2003)
JtTr LG:
JtTr cM:
Km/O41 map info (Nakano et al. 2003)
Km/O41 LG:
Km/O41 cM:
Rp/Sa map info (Ohta et al. 2011)
Rp/Sa LG: RP-08
Rp/Sa cM: 15.967
CAPS genotyping (Hybrid cv/Citrus collection)
CAPS genotyping:
Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)
Cultivar identification M/T/K:
SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)
SNP1: SI240
DNA sequence around SNP1:
TGTTGTTATTGAGACAAAAATGTAGGAGGATTTGGAGAACATATGCATAATTCAGGGAGG [A/G]GCAGTGTCGTAGTTACAGAACAAATTCGAGGCCAATTAAT |
SNP2:
DNA sequence around SNP2:
SNP3:
DNA sequence around SNP3:
SNP4:
DNA sequence around SNP4: