Locus name: Fb0301
CAPS PCR info
PCR Anneal-Temp (℃) / Method: 58Main product (bp): 1400
Comment on PCR:
F-primer: ATTTACGGCTTATTCTTTTGC
R-primer: CGCGCCTTATTTTCTTGTA
EST info
EST Accession number 1: DC888236EST Accession number 2: DC888237
sequence-tagged site (STS seq)
STS:| ACCCAAGTTAACAGTTGTATAGGAAGCACCTGTCTTGCCACAAATTCCATTATTTCTGCC TCATTGAGACTGCTGTGCGGCTGCCTTACCACAAAAGCCATGGGCACCTGACCAGCTTCT TCATCAGGATATCTGCAAATATGAATTTTCTGTTAATCAGAATTTACTCTATATTCTTAG CCGAACAACACAATGGCTATTATAAGAATTCAAGGAAAAATAAATAAATAAGGGCTTATA AACTAGTTACGGAATGACTGCAGCGTCAGCAACCTCCGGATGGGACAGAAGCAAGTGTTC TAGTTCTGCAGGGGCAACCTACACAATGTAACAACAACGGAAATGGAAATGGAAATATTA GCTGTATGATCTTAACAAACCAAATCAATAGAGGAAAAACA |
STS Forward / Reverse:
Experiments: Restriction fragment profile
Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)Comment:
Figure legend:
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Compared cultivars (Left, (Middle), Right): Frying dragon/Hyuuganatsu
Comment:
Figure legend:
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Compared cultivars (Left, (Middle), Right): Trovita orange/Kiyomi/Miyagawa-wase
Comment:
Figure legend:
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Experiments: CAPS pattern of hybrid cultivar
Resriction enzyme: Hinc2Rank as CAPS marker: Available marker
Comment:
Figure legend:
CVName 1. Nagami, 2. Budha's, 3. Miyagawa, 4. Trovita, 5. Kiyomi, 6. Tachibana, 7. Koji, 8. Kinokuni (8' Hirakishu), 9. Mato pumello, 10. Kunip,
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)
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Experiments: CAPS pattern of Citrus collection
Experiments: Segregation pattern
Population name: Kankitsu Chuukanbohon Nou8gou x Siamese AcidlessRestriction enzyme: EcoR1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
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Target on C. clementina (v1.0)
Transcript ID: Ciclev10014804mScaffold: scaffold_2
Start: 14143537
End: 14146003
Target on C. unshiu (v1.0)
Transcript ID: C_unshiu_00466:mRNA_1.1Scaffold: C_unshiu_00466
Start: 13811
End: 16178
Gene function: 4-coumarate:coenzyme A ligase 4
AGI map info (Shimada et al. 2014)
AGI LG: AGI-06AGI cM: 92.727
KmMg map info (Omura et al. 2003)
KmMg LG: KmG-06KmMg cM:
JtTr map info (Omura et al. 2003)
JtTr LG:JtTr cM:
Km/O41 map info (Nakano et al. 2003)
Km/O41 LG:Km/O41 cM:
Rp/Sa map info (Ohta et al. 2011)
Rp/Sa LG: RP-06.1Rp/Sa cM: 16.627
CAPS genotyping (Hybrid cv/Citrus collection)
CAPS genotyping: HbCultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)
Cultivar identification M/T/K:SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)
SNP1: SI053DNA sequence around SNP1:
| CAGCAACCTCCGGATGGGACAGAAGCAAGTGTTCTAGTTCTGCAGGGGCAACCTACACAA [T/C]GTAACAACAACGGAAATGGAAATGGAAATATTAGCTGTATGATCTTAACAAACCA AATCA |
DNA sequence around SNP2:
DNA sequence around SNP3:
DNA sequence around SNP4: