Citrus CAPS Marker Database

Locus name: Fb0357

CAPS PCR info

PCR Anneal-Temp (℃) / Method: 60
Main product (bp): 1400
Comment on PCR:
F-primer: AGAGGGGAACAACAGTCTT
R-primer: TTTCTGATAGCCAAAATGGTA

EST info

EST Accession number 1: DC888303
EST Accession number 2: DC888304

sequence-tagged site (STS seq)

STS:

GCATATTGGTACAGATAAGAATTCTGTTAGTATTGCAACGTGCTGTCTGCCAAAAATTAG
CCATGAAAAATCAGGTAAAGCTGTCAATTGATCATATGTTGGGATTTTCTAGTTGTGATC
CGTTATGCAAGGGGCAATGCTGTACTTCATGTGTCTTCAATTAGATTCTTTTAAGTGAAT
TTATAGGTAAGATGAGCGCTTTGTATTTATTTTTACCATTCTTTTTGAGTGTACCTCTTT
TCTCTTTTCTCTTCTCTTTTT

STS source:
STS Forward / Reverse:

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:

Compared cultivars (Left, (Middle), Right): Frying dragon/Hyuuganatsu
Comment:
Figure legend:

Compared cultivars (Left, (Middle), Right): Shiamese acidless/RP-94
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Experiments: CAPS pattern of Citrus collection

Rsetriction enzyme: Msp1
Comment:
Figure legend:

CV (No.1 Top) 101 Tahiti, 100 Rangpurlime, 102 Limonia, 104 LisbonL, 99 SweetLime, 92 Hirado, 87 MGF, 85 Kinukawa, 84 Hassaku, 83 Yamamikan, 78 Natsudaidai, 58 Rokugatu, Tr Trovita, 50 WNV, 44 Tankan, 42 Iyo,
(No.1 Bottom) 39 HgNt, 36 Ujukitsu, 32 Kawabata, 30 Shunkokan, 70 Yuzu, 54 Henka, 53 Ichang lemon, 37 Kunenbo, Mg Miyagawa, 31 Yatsushiro, 27 Kabuchii, 26 Ponkan, 25 Medet. 22. Dancy, 14 Tachibana, 11 Hirakishu (26' Ohta ponkan)

(No.2 Top) 6 Cleopatra, 4 CQ, 2 Fukuremikan, FD Hiryuu, 124 Largeleaf, 125 Small leaf, 141 CTV-S, 136 Rubidoaux,
(No.2 Bottom) FD, Hassaku, Km, F1, RP94, SA, 02, 04, 05, 06, 07, 08, 09, 10, 11, 13

BDZ BenDi Zao, SO Shuto, BP Banpeiyu

Experiments: Segregation pattern

Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Bcl2
Comment:
Figure legend:

A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless

Population name: Kankitsu Chuukanbohon Nou8gou x Siamese Acidless
Restriction enzyme: Msp1
Comment:
Figure legend:

Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Pst1
Comment:
Figure legend:

Population name: Kankitsu Chuukanbohon Nou8gou x Siamese Acidless
Restriction enzyme: Sty1
Comment:
Figure legend:

Target on C. clementina (v1.0)

Transcript ID: Ciclev10001633m
Scaffold: scaffold_5
Start: 40550222
End: 40554434

Target on C. unshiu (v1.0)

Transcript ID: C_unshiu_00889:mRNA_6.1
Scaffold: C_unshiu_00889
Start: 44175
End: 51825
Gene function: Hypothetical protein

AGI map info (Shimada et al. 2014)

AGI LG: AGI-09
AGI cM: 39.104

KmMg map info (Omura et al. 2003)

KmMg LG:
KmMg cM:

JtTr map info (Omura et al. 2003)

JtTr LG:
JtTr cM:

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG:
Km/O41 cM:

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG:
Rp/Sa cM:

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping:

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K:

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1: SI384
DNA sequence around SNP1:

ATTGATCATATGTTGGGATTTTCTAGTTGTGATC[T/C]GTTATGCAAGGGGCAATGCTG
TACTTCATGTGTCTTCAATTAGATTCTTTTAAGTGAATT

SNP2:
DNA sequence around SNP2:

SNP3:
DNA sequence around SNP3:

SNP4:
DNA sequence around SNP4: