Citrus CAPS Marker Database

Locus name: Fb0364

CAPS PCR info

PCR Anneal-Temp (℃) / Method: 62
Main product (bp): 2200
Comment on PCR: Additional bands
F-primer: TTATCAAAATGTCGCCACCAG
R-primer: CAATGTCCCGAGGCTCAA

EST info

EST Accession number 1: DC888316
EST Accession number 2:

sequence-tagged site (STS seq)

STS:
CTGGGAGCTCAAATCCTCTATTTTGGCTTCCCCAAATATGACGTACGTTTAAGAATTTGG
GCTCTTGAAAACATCAGGCTTTGAGATGAAAAATAGTATCTGCAAACTCAGAAAAAAGAG
AGAAAAAAAGGAAAAAAGAAAAAATTGATAACCATCCATCAACTAAGCAAAATAACTTCT
TAACAAATATAATAACATAAAAAGCCTTACATTTTTGGTTCTCTTGATGGTTACCCTGCT
AACTCCTGTTACAGGTTTCATGCCCAACTTCAGCATTGCCTTGCGGCTCTTTTTCTCACT
TCTGCTCTGCTTTGAACTCCCTCCTGCAACTCAGTAACCAATCAAGACTACTTAAATCAC
TGTAGAAACTACATCCATTGCATTTCAACCTTCAAACAAAAATGACAACATAATTCCATA
T
STS source:
STS Forward / Reverse:

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:
Compared cultivars (Left, (Middle), Right): Frying dragon/Hyuuganatsu
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Resriction enzyme: Nde2
Rank as CAPS marker: Available marker
Comment:
Figure legend:
CVName 1. Nagami, 2. Budha's, 3. Miyagawa, 4. Trovita, 5. Kiyomi, 6. Tachibana, 7. Koji, 8. Kinokuni (8' Hirakishu), 9. Mato pumello, 10. Kunip,
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)

Experiments: CAPS pattern of Citrus collection

Experiments: Segregation pattern

Population name: Kankitsu Chuukanbohon Nou8gou x Siamese Acidless
Restriction enzyme: Nde2
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Nde2
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: Jutaro unshu x Trovita orange
Restriction enzyme: Nde2
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: Kiyomi x Miyagawa-wase
Restriction enzyme: Nde2
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless

Target on C. clementina (v1.0)

Transcript ID: Ciclev10022215m
Scaffold: scaffold_3
Start: 13810497
End: 13813004

Target on C. unshiu (v1.0)

Transcript ID: C_unshiu_00542:mRNA_3.1
Scaffold: C_unshiu_00542
Start: 15940
End: 18433
Gene function: Nascent polypeptide-associated complex subunit alpha-like protein

AGI map info (Shimada et al. 2014)

AGI LG: AGI-05
AGI cM: 41.306

KmMg map info (Omura et al. 2003)

KmMg LG: KmG-05S
KmMg cM: 55.482

JtTr map info (Omura et al. 2003)

JtTr LG: JtR-05
JtTr cM: 39.609

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG:
Km/O41 cM:

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG: RP-05
Rp/Sa cM: 56.834

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping: Hb

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K:

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1: SI373
DNA sequence around SNP1:
GCCCAACTTCAGCATTGCCTTGCGGCTCTTTTTCTCACTTCTGCTCTGCTTTGAACTCCC
[A/T]CCTGCAACTCAGTAACCAATCAAGACTACTTAAATCACTGTAGAAACTACATCCA
TTGCA
SNP2:
DNA sequence around SNP2:
SNP3:
DNA sequence around SNP3:
SNP4:
DNA sequence around SNP4: