Citrus CAPS Marker Database

Locus name: Fb2159

CAPS PCR info

PCR Anneal-Temp (℃) / Method: 58
Main product (bp): 1400
Comment on PCR:
F-primer: GGTGGCCAAGAAAGTTC
R-primer: CTAAAACGTTATGGGCTGATA

EST info

EST Accession number 1: DC890329
EST Accession number 2: DC890330

sequence-tagged site (STS seq)

STS:
TCTGGGATGCTCTGGTGAATTTGAGCTCTGGGTTTCAAAGTAAAATCAAGAAACATCGTC
GGTGTTCTTGTGTAAACCGGAGACCGGACTCCCATCGATTCTCCGGCTATTATTCGGACT
TCGACCCCATCTGTCTCTGCCCTTTATATCTCCTCGCTTGGAATTTCTTGATACCTCGGT
TCAATCCTGCAACAATAAAATGCATATCTTAGTGGTGAAATTATGAGCAGAAGCAATAAA
ACCTTGAGAGCTTTTTGAAGTTGGCGTTTGTTTCATACATTTTGTCCCTGGAAGATAGAT
TGATCCAAAGTTGTAAACCATTTTGTACGCCTTCACCGGCAGGCATTTCGGAGTGGACTA
TTCCTCTTCCTGCTGTCATCC
STS source:
STS Forward / Reverse:

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:
Compared cultivars (Left, (Middle), Right): Frying dragon/Hyuuganatsu
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Experiments: CAPS pattern of Citrus collection

Experiments: Segregation pattern

Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Dra1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: Jutaro unshu x Trovita orange
Restriction enzyme: Dra1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: Kiyomi x Miyagawa-wase
Restriction enzyme: Dra1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: Kankitsu Chuukanbohon Nou8gou x Siamese Acidless
Restriction enzyme: Rsa1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless

Target on C. clementina (v1.0)

Transcript ID: Ciclev10021136m
Scaffold: scaffold_3
Start: 39606431
End: 39608387

Target on C. unshiu (v1.0)

Transcript ID: C_unshiu_00037:mRNA_76.1
Scaffold: C_unshiu_00037
Start: 551437
End: 553057
Gene function: Pirin family protein

AGI map info (Shimada et al. 2014)

AGI LG: AGI-05
AGI cM: 49.856

KmMg map info (Omura et al. 2003)

KmMg LG: KmG-05S
KmMg cM: 68.781

JtTr map info (Omura et al. 2003)

JtTr LG: JtR-05
JtTr cM: 41.77

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG:
Km/O41 cM:

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG: SA-09
Rp/Sa cM:

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping:

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K:

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1: SI205
DNA sequence around SNP1:
GATTCTCCGGCTATTATTCGGACTTCGACCCCATCTGTCTCTGCCCTTTATATCTCCTCG
[C/G]TTGGAATTTCTTGATACCTCGGTTCAATCCTGCAACAATAAAATGC
SNP2: SI300
DNA sequence around SNP2:
TCTGGGATGCTCTGGTGAATTTGAGC[T/C]CTGGGTTTCAAAGTAAAATCAAGAAACAT
CGTCGGTGTTCTTGTGTAAACCGGAGACCGG
SNP3:
DNA sequence around SNP3:
SNP4:
DNA sequence around SNP4: