Locus name: Gn0021
CAPS PCR info
PCR Anneal-Temp (℃) / Method: 55Main product (bp): 1000
Comment on PCR:
F-primer: GCCCAAGATGTCGGAAGTTGT
R-primer: AATGAATACAATGCTGAAGGGC
EST info
EST Accession number 1: AB115767EST Accession number 2:
sequence-tagged site (STS seq)
Experiments: Restriction fragment profile
Compared cultivars (Left, (Middle), Right): Trovita orange/Kiyomi/Miyagawa-waseComment: PCR_condition: 56C aneal
Figure legend:
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Experiments: CAPS pattern of hybrid cultivar
Resriction enzyme: Hae3Rank as CAPS marker: Available marker
Comment: PCR_condition: 56C aneal
Figure legend:
CVName 1. Nagami, 2. Budha's, 3. Miyagawa, 4. Trovita, 5. Kiyomi, 6. Tachibana, 7. Koji, 8. Kinokuni (8' Hirakishu), 9. Mato pumello, 10. Kunip,
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)
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Experiments: CAPS pattern of Citrus collection
Experiments: Segregation pattern
Population name: Kiyomi x Okitsu41gouRestriction enzyme: Hae3
Comment: PCR_condition: 56C aneal
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
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Target on C. clementina (v1.0)
Transcript ID: Ciclev10000597mScaffold: scaffold_5
Start: 39630111
End: 39635609
Target on C. unshiu (v1.0)
Transcript ID: C_unshiu_01033:mRNA_10.1Scaffold: C_unshiu_01033
Start: 47799
End: 53166
Gene function: Somatic embryogenesis receptor kinase 1
AGI map info (Shimada et al. 2014)
AGI LG: AGI-09AGI cM: 42.621
KmMg map info (Omura et al. 2003)
KmMg LG: KmG-09KmMg cM: 68.22
JtTr map info (Omura et al. 2003)
JtTr LG: JtR-09SJtTr cM: 97.453
Km/O41 map info (Nakano et al. 2003)
Km/O41 LG: NAT-09Km/O41 cM: 83.381
Rp/Sa map info (Ohta et al. 2011)
Rp/Sa LG:Rp/Sa cM:
CAPS genotyping (Hybrid cv/Citrus collection)
CAPS genotyping: HbCultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)
Cultivar identification M/T/K:SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)
SNP1:DNA sequence around SNP1:
DNA sequence around SNP2:
DNA sequence around SNP3:
DNA sequence around SNP4: