Citrus CAPS Marker Database

Locus name: Gn0021


CAPS PCR info

PCR Anneal-Temp (℃) / Method: 55
Main product (bp): 1000
Comment on PCR:
F-primer: GCCCAAGATGTCGGAAGTTGT
R-primer: AATGAATACAATGCTGAAGGGC

EST info

EST Accession number 1: AB115767
EST Accession number 2:

sequence-tagged site (STS seq)


Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): Trovita orange/Kiyomi/Miyagawa-wase
Comment: PCR_condition: 56C aneal
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Resriction enzyme: Hae3
Rank as CAPS marker: Available marker
Comment: PCR_condition: 56C aneal
Figure legend:
CVName 1. Nagami, 2. Budha's, 3. Miyagawa, 4. Trovita, 5. Kiyomi, 6. Tachibana, 7. Koji, 8. Kinokuni (8' Hirakishu), 9. Mato pumello, 10. Kunip,
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)

Experiments: CAPS pattern of Citrus collection


Experiments: Segregation pattern

Population name: Kiyomi x Okitsu41gou
Restriction enzyme: Hae3
Comment: PCR_condition: 56C aneal
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless

Target on C. clementina (v1.0)

Transcript ID: Ciclev10000597m
Scaffold: scaffold_5
Start: 39630111
End: 39635609

Target on C. unshiu (v1.0)

Transcript ID: C_unshiu_01033:mRNA_10.1
Scaffold: C_unshiu_01033
Start: 47799
End: 53166
Gene function: Somatic embryogenesis receptor kinase 1

AGI map info (Shimada et al. 2014)

AGI LG: AGI-09
AGI cM: 42.621

KmMg map info (Omura et al. 2003)

KmMg LG: KmG-09
KmMg cM: 68.22

JtTr map info (Omura et al. 2003)

JtTr LG: JtR-09S
JtTr cM: 97.453

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG: NAT-09
Km/O41 cM: 83.381

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG:
Rp/Sa cM:

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping: Hb

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K:

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1:
DNA sequence around SNP1:
SNP2:
DNA sequence around SNP2:
SNP3:
DNA sequence around SNP3:
SNP4:
DNA sequence around SNP4: