Citrus CAPS Marker Database

Locus name: Lp0226

CAPS PCR info

PCR Anneal-Temp (℃) / Method: 56
Main product (bp): 2000
Comment on PCR:
F-primer: CCAACGCAAGGAAGATAGA
R-primer: CTCAACAGTTAAACCCAAAAA

EST info

EST Accession number 1: AU300802
EST Accession number 2: DC893303

sequence-tagged site (STS seq)

STS:

TTGCTTTCTTTCTGAGTAATCCTCTCTGGCACTTTGTAATAGTCCGGGGAAGTCTGATAT
TTATGACTTCTTTAGAGCAGCCTAGATAATATTTTAATTCATTTTTACCAACTAAAATAA
CTGAGGTTTGTTTGGCATTCCAATTTTGTAGATGCTGGGAGAGAATCTCTACCCACTTGT
AGAACAGCTGGAGCCTGATGCTGCAGCTAAGGTTACGGGAATGCTTTTGGAGATGGATCA
GACTGAGGTTTTGCACTTGCTCGAGTCACCAGAAGCTCTGAAAGCGAAAGTGGCTGAGGC
AATGGAGGTTCTGAGGAATGTTGCTCA

STS source:
STS Forward / Reverse:

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:

Compared cultivars (Left, (Middle), Right): Frying dragon/Hyuuganatsu
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Experiments: CAPS pattern of Citrus collection

Rsetriction enzyme: Msp1
Comment:
Figure legend:

CV (No.1 Top) 101 Tahiti, 100 Rangpurlime, 102 Limonia, 104 LisbonL, 99 SweetLime, 92 Hirado, 87 MGF, 85 Kinukawa, 84 Hassaku, 83 Yamamikan, 78 Natsudaidai, 58 Rokugatu, Tr Trovita, 50 WNV, 44 Tankan, 42 Iyo,
(No.1 Bottom) 39 HgNt, 36 Ujukitsu, 32 Kawabata, 30 Shunkokan, 70 Yuzu, 54 Henka, 53 Ichang lemon, 37 Kunenbo, Mg Miyagawa, 31 Yatsushiro, 27 Kabuchii, 26 Ponkan, 25 Medet. 22. Dancy, 14 Tachibana, 11 Hirakishu (26' Ohta ponkan)

(No.2 Top) 6 Cleopatra, 4 CQ, 2 Fukuremikan, FD Hiryuu, 124 Largeleaf, 125 Small leaf, 141 CTV-S, 136 Rubidoaux,
(No.2 Bottom) FD, Hassaku, Km, F1, RP94, SA, 02, 04, 05, 06, 07, 08, 09, 10, 11, 13

BDZ BenDi Zao, SO Shuto, BP Banpeiyu

Rsetriction enzyme: Pvu2
Comment:
Figure legend:

(No.2 Top) 6 Cleopatra, 4 CQ, 2 Fukuremikan, FD Hiryuu, 124 Largeleaf, 125 Small leaf, 141 CTV-S, 136 Rubidoaux,
(No.2 Bottom) FD, Hassaku, Km, F1, RP94, SA, 02, 04, 05, 06, 07, 08, 09, 10, 11, 13

BDZ BenDi Zao, SO Shuto, BP Banpeiyu

Experiments: Segregation pattern

Population name: Kankitsu Chuukanbohon Nou8gou x Siamese Acidless
Restriction enzyme: Pvu2
Comment:
Figure legend:

A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless

Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Pvu2
Comment:
Figure legend:

Target on C. clementina (v1.0)

Transcript ID: Ciclev10027999m
Scaffold: scaffold_8
Start: 2831832
End: 2837109

Target on C. unshiu (v1.0)

Transcript ID: C_unshiu_00152:mRNA_19.1
Scaffold: C_unshiu_00152
Start: 138714
End: 144356
Gene function: Embryonic polyadenylate-binding protein

AGI map info (Shimada et al. 2014)

AGI LG: AGI-08
AGI cM: 73.527

KmMg map info (Omura et al. 2003)

KmMg LG:
KmMg cM:

JtTr map info (Omura et al. 2003)

JtTr LG:
JtTr cM:

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG:
Km/O41 cM:

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG:
Rp/Sa cM:

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping:

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K:

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1: SI210
DNA sequence around SNP1:

AGCTAAGGTTACGGGAATGCTTTTGGAGATGGATCAGACTGAGGTTTTGCACTTGCTCGA
[A/G]TCACCAGAAGCTCTGAAAGCGAAAGTGGCTGAGGCAATGGAGGTTCTGAGGAATG
TTGCT

SNP2:
DNA sequence around SNP2:

SNP3:
DNA sequence around SNP3:

SNP4:
DNA sequence around SNP4: