Citrus CAPS Marker Database

Locus name: Mf0017

CAPS PCR info

PCR Anneal-Temp (℃) / Method: 60
Main product (bp): 1500
Comment on PCR:
F-primer: TTCTTGGGCTGCTGTTGTTGGGGA
R-primer: AAAGTCACAAGACATAACATTGAT

EST info

EST Accession number 1: C81722
EST Accession number 2: DC893598

sequence-tagged site (STS seq)

STS:

ACAGCAGCTCTGAGCACTGCGCTTTTCAACAATGGCATGAGCTGTGGGGCTTGTTTTGAG
ATCAAGTGTGTTAATGACCCAAGATGGTGCCGTCCGGGCTCCATTTTTGTTACTGCCACA
AATTTTTGCCCTCCAAATAAT

STS source: Okitsu 46
STS Forward / Reverse: Foward read

STS:

TCCTTGACAAGAAAAGACACAACAATACAATTGAAAATATATATATATATAGAGATGGCG
GGTGGTACATGCCCTCCACTGCCAGCCAATTTCTGAATA

STS source: Chuukanbohon NO 5
STS Forward / Reverse: Reverse read

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:

Compared cultivars (Left, (Middle), Right): Miyagawa-wase/Trovita orange
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Resriction enzyme: Hha1
Rank as CAPS marker: Others
Comment:
Figure legend:

CVName 1. Nagami, 2. Budha's, 3. Miyagawa, 4. Trovita, 5. Kiyomi, 6. Tachibana, 7. Koji, 8. Kinokuni (8' Hirakishu), 9. Mato pumello, 10. Kunip,
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)

Resriction enzyme: Pvu2
Rank as CAPS marker: no homozygous cultivar with minor allele
Comment:
Figure legend:

Experiments: CAPS pattern of Citrus collection

Experiments: Segregation pattern

Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Hha1
Comment:
Figure legend:

A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless

Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Pvu2
Comment:
Figure legend:

Target on C. clementina (v1.0)

Transcript ID: Ciclev10021897m
Scaffold: scaffold_3
Start: 7337405
End: 7339222

Target on C. unshiu (v1.0)

Transcript ID: C_unshiu_00199:mRNA_11.1
Scaffold: C_unshiu_00199
Start: 77240
End: 79028
Gene function: Expansin

AGI map info (Shimada et al. 2014)

AGI LG: AGI-05
AGI cM: 44.674

KmMg map info (Omura et al. 2003)

KmMg LG:
KmMg cM:

JtTr map info (Omura et al. 2003)

JtTr LG:
JtTr cM:

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG:
Km/O41 cM:

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG:
Rp/Sa cM:

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping: Hb

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K:

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1:
DNA sequence around SNP1:

SNP2:
DNA sequence around SNP2:

SNP3:
DNA sequence around SNP3:

SNP4:
DNA sequence around SNP4: