Citrus CAPS Marker Database

Locus name: Mf0057

CAPS PCR info

PCR Anneal-Temp (℃) / Method: 56
Main product (bp): 1400
Comment on PCR: Additional bands
F-primer: GGATTTCTCAACAAGATCAAG
R-primer: AAAGTGAATCCACCAGCACAG

EST info

EST Accession number 1: C81818
EST Accession number 2: DC893630

sequence-tagged site (STS seq)

STS:
AGTATTCTGAGTTGTCCGATAAGCCCAAAGAACGCTGGGTAGCTCTTCTATCAAAGCTCC
CTTCCTTTCCTCTAGCCTATTCTTTAAGATACCCTTGATGATTTTGTTGATGGCTTCCAC
CTGTCCATTAGATTGCGGGTGAGCCAGAGAAGCAAATCGGTTGGCTATCCCAAGCTCCGA
GCAGAAACTCTGAAATTTTTCATTATCGAATTGCTTCCCGTTATTGCTCACAATTGCGTT
AGGATTACCATATCTGCATATGAGGTTCCTCCAAACGAAATCAGTAGTTTTTCTCTCGGT
AATGCTTGCCAACAGCTCCGCTTCTACCCACTTTGTTTAAAGACATCATTTTCTTTTCTA
ATTCACTCCATTGACTTGAGAATTTTGGAATATCATCTCCTATTTCATTTTTAGTCATAG
GTATAAATGAACCATCATAAATAACTTCCCAAATTTCTTAATCTAAAGCTTGCAAATAAA
TTCTCATCTTAGTTTTCCAATATGGGTAGTCATTACCATTAAAGAATGAAGATCTAGTGG
TGGATTGGCCTTCCATGAAGGTTGAATTCGGGNTTGCTATGGATCTTTAAGTCTATATGG
TTAAGTCCACACAAGAGCACCTCGCTCTGATACCAAATGAATACTAACTAGGTAACCTAA
GAAGTGAGGGATTAGGATTTACCATACAAGTTTTTGCTATCAAACTAGCCAACACTATTT
TTGGACCAA
STS source: Okitsu 46
STS Forward / Reverse: Foward read
STS:
AAAGAACGCTGGGTAGCTCTTCTATCAAAGCTCCCTTCCTTTCCTCTAGCCTATTCTTTA
AGATACCCTTGATGATTTTGTTGATGGCTTCCACCTGTCCATTAGATTGCGGGTGAGCCA
GAGAAGCAAATCGGTTGGCTATCCCAAGCTCCGAGCAGAAACTCTGAAATTTTTCATTAT
CGAATTGCTTCCCGTTATTGCTCACAATTGCGTTAGGATTACCATATCTGCATATGAGGT
TCCTCCAAACGAAATCAGTAGTTTTTCTCTCGGTAATGCTTGCCAACAGCTCCGCTTCTA
CCCACTTTGTTTAAAGACATCATTTTCTTTTCTAATTCACTCCATTGACTTGAGAATTTT
GGAATATCATCTCCTATTTCATTTTTAGTCATAGGTATAAATGAACCATCATAAATAACT
TCCCAAATTTCTTAATCTAAAGCTTGCAAATAAATTCTCATCTTAGTTTT
STS source:
STS Forward / Reverse: Foward read
STS:
AGACATCATTTTCTTTTCTAATTCACTCCATTGACTTGAGAATTTTGGAATATCATCTCC
TATTTCATTTTTAGTCATAGGTATAAATGANCCCATCATAAATAACTTCCCAAATTTCTT
AATCTAAAGCTTGCAAATAAATTCTCATCTTAGTTTTCCAATATGGGTAGTCATTACCAT
TAAAGAATGAAGATCTAGTGGTGGATTGGCCTTCCATGAAGGTTGAATTCGGGTTGCTAT
GGATCTTTAAGTCTATATGGTTAAGTCCACACAAGAGCACCTCGCTCTGATACCAAATGA
AATACTAACTAGGTAACCTAAGAAGTGAGTGAATTAGGATTTTAACCATACAAGTTTTTT
GCTATCAAAACTAGCCAAACACTAATTTTTTGGACCAAACTGCCCCTATCATTTTCACTC
AAGCTCAC
STS source: Okitsu 46
STS Forward / Reverse: Reverse read

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): Miyagawa-wase/Trovita orange/Kiyomi
Comment:
Figure legend:
Compared cultivars (Left, (Middle), Right): Trovita orange/Kiyomi/Miyagawa-wase
Comment:
Figure legend:
Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Resriction enzyme: Hinc2
Rank as CAPS marker: Others
Comment:
Figure legend:
CVName 1. Nagami, 2. Budha's, 3. Miyagawa, 4. Trovita, 5. Kiyomi, 6. Tachibana, 7. Koji, 8. Kinokuni (8' Hirakishu), 9. Mato pumello, 10. Kunip,
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)

Experiments: CAPS pattern of Citrus collection

Experiments: Segregation pattern

Population name: Kankitsu Chuukanbohon Nou8gou x Siamese Acidless
Restriction enzyme: NC
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless

Target on C. clementina (v1.0)

Transcript ID: Ciclev10002349m
Scaffold: scaffold_5
Start: 42489684
End: 42491501

Target on C. unshiu (v1.0)

Transcript ID: No
Scaffold:
Start:
End:
Gene function:

AGI map info (Shimada et al. 2014)

AGI LG:
AGI cM:

KmMg map info (Omura et al. 2003)

KmMg LG:
KmMg cM:

JtTr map info (Omura et al. 2003)

JtTr LG:
JtTr cM:

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG:
Km/O41 cM:

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG:
Rp/Sa cM:

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping: Hb

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K:

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1:
DNA sequence around SNP1:
SNP2:
DNA sequence around SNP2:
SNP3:
DNA sequence around SNP3:
SNP4:
DNA sequence around SNP4: