Citrus CAPS Marker Database

Locus name: Mf0084

CAPS PCR info

PCR Anneal-Temp (℃) / Method: 56
Main product (bp): 700
Comment on PCR:
F-primer: TGCTGAATGAGGATGAGTTGA
R-primer: GCAACGCTACAGACTAAAGAG

EST info

EST Accession number 1: C81872
EST Accession number 2:

sequence-tagged site (STS seq)

STS:
TTTGCTGAATGAAGGATGAGTTGAGGGATGCTGTGCTGCTTGTTTTTGCCAACAAGCAAG
ATCTTCCAAATGCCATGAATGCTGCAGAGATCACTGATAAGCTTGGTCTTCACTCACTTC
GTCAACGCCACTGGTACTACAAATACTCCTAGTCTCTCTCTACCGGACTTATGTTTTGAT
GGTTATTGACAAGAGNTGGCATTTTGTGTGTTCCTTTCAGGTACATCCAGAGCACATGTG
CAACATCTGGGGAAGGACTCTATGAGGGTCTGGACTGGCTCTCCAACAACATCGCTAACA
AGGTGGGGTTATGATTTCATTTGTTATGTACACCGATCTTTTATATCAATTACCTTGCTT
AACNGTTCTNGTTAAATTTCATCATCATGTGCAGGCTTAANTGATTGGTCAAGTCTGAGC
CACTCTTTGCGGCAGCATCTATGTGAATGATGTTACTATGCTTTTGTTTATTTTGTCGGG
GATTCTTTTTGGGCTTTACTACCTACGTTCAGAAGAATGTATTTGTTTGTGTTAGATATT
TGATTGGCACCATACTATAAAGTTTTCTGCANTGNC
STS source:
STS Forward / Reverse: Reverse read
STS:
CAATCAAATATCTAACACAAACAAATACATTCTTCTGAACGTAGGTAGTAAAGCCCAAAA
AGAATCCCCGACAAAATAAACAAAAGCATAGTAACATCATTCACATAGATGCTGCCGCAA
AGAGTGGCTCAGACTTGACCAATCACTTAAGCCTGCACATGATGATGAAATTTAACNAGA
ACTGTTAAGCAAGGTAATTGATATAAAAGATCGGTGTACATAACAAATGA
STS source:
STS Forward / Reverse: Reverse read

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): Trovita orange/Kiyomi/Miyagawa-wase
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Resriction enzyme: Hae3
Rank as CAPS marker: Others
Comment:
Figure legend:
CVName 1. Nagami, 2. Budha's, 3. Miyagawa, 4. Trovita, 5. Kiyomi, 6. Tachibana, 7. Koji, 8. Kinokuni (8' Hirakishu), 9. Mato pumello, 10. Kunip,
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)
Resriction enzyme: Mbo1
Rank as CAPS marker: Others
Comment:
Figure legend:
CVName 1. Nagami, 2. Budha's, 3. Miyagawa, 4. Trovita, 5. Kiyomi, 6. Tachibana, 7. Koji, 8. Kinokuni (8' Hirakishu), 9. Mato pumello, 10. Kunip,
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)
Resriction enzyme: Nde2
Rank as CAPS marker: Others
Comment:
Figure legend:
CVName 1. Nagami, 2. Budha's, 3. Miyagawa, 4. Trovita, 5. Kiyomi, 6. Tachibana, 7. Koji, 8. Kinokuni (8' Hirakishu), 9. Mato pumello, 10. Kunip,
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)

Experiments: CAPS pattern of Citrus collection

Experiments: Segregation pattern

Population name: Jutaro unshu x Trovita orange
Restriction enzyme: Mbo1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: Kiyomi x Miyagawa-wase
Restriction enzyme: Mbo1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: Kiyomi x Miyagawa-wase
Restriction enzyme: NC
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Nde2
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: Kiyomi x Okitsu41gou
Restriction enzyme: Nde2
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless

Target on C. clementina (v1.0)

Transcript ID: Ciclev10012898m
Scaffold: scaffold_6
Start: 40381
End: 43868

Target on C. unshiu (v1.0)

Transcript ID: C_unshiu_01371:mRNA_1.1
Scaffold: C_unshiu_01371
Start: 17746
End: 21242
Gene function: ADP-ribosylation factor A1F

AGI map info (Shimada et al. 2014)

AGI LG: AGI-04
AGI cM: 114.409

KmMg map info (Omura et al. 2003)

KmMg LG: KmG-04
KmMg cM: 66.806

JtTr map info (Omura et al. 2003)

JtTr LG: JtR-04
JtTr cM: 11.413

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG:
Km/O41 cM:

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG:
Rp/Sa cM:

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping:

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K:

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1: SI383
DNA sequence around SNP1:
CAATCAAATATCTAACACAAACAAATACATTCT[A/T]CTGAACGTAGGTAGTAAAGCCC
AAAAAGAATCCCCGACAAAATAAACAAAAGCATAGTAA
SNP2:
DNA sequence around SNP2:
SNP3:
DNA sequence around SNP3:
SNP4:
DNA sequence around SNP4: