Citrus CAPS Marker Database

Locus name: Ov0128

CAPS PCR info

PCR Anneal-Temp (℃) / Method: 58
Main product (bp): 1600
Comment on PCR:
F-primer: CAGGCCGAGGACATTATCA
R-primer: TCCCACATCTACATTGCCATT

EST info

EST Accession number 1: AU186447
EST Accession number 2: DC893859

sequence-tagged site (STS seq)

STS:
ATGTTTTTATGTTGATGATACTGTTGTTTCCTCTCATCGGTCACCAGTGAGTTAATGCAT
AGGGACAGGTCAGCTCTTTTAAATTATAAATTCATTTTTTGGCCGTTAGCGTTGCTACCA
GCGNATGTACATATGAATGTGTCTTTTGCCTTGTAACAATGNGTCTCTTGAAGGTTGAGG
GTGGTCGACCATAGAGTGAAGCATATTGTTTGCCTTTTGTGGTTTTCAAAAGTAAAGCCT
TTTTGTGCATTTGAAGTCTTAGCCTAGTTGTGCAAGGGAGCTGCCCATGGGCTGGGCAGG
TTGCTGGGTTTCAATTNTTAAGTCATGGCAAAGACAGGATTGAAAGGTGTGATCCTGTTT
CCTATATATTGTTCGAGCTTCTGACACATATAATTTTGGTCTCCCAGATCCGACCTTTGT
GGAGACATTATTTCCAAAACACCCAAGGACTCATCTTTGTGGTTGACAGTAATGATCGCG
ACCGTGTGGTAGAAGCTAGGGATGAGTTGCACAGAATGTTGAATGAGGTAAACTATTGCT
ACTAAAAGGAAACAATAGCTNTAGTTGCAGAATGTTTGTTTTAGTGGAATTCTCATTTTT
TACTGGAATTTGGCAAGGTGGTTCAGTGTCTTGATTGTAATGTTTTTTTTGTTGCATTAA
CAGGATGAATTGAGAGATGCTGTGCTGCTTGTATTTGCTAACAAGCAAGATCTTCCAAAT
GCAATGAATGCTGCTGAAA
STS source: Okitsu 46
STS Forward / Reverse: Foward read
STS:
CACATGTGCCACTTCTGGTGAAGGGCTGTATGAGGGACTTGACTGGCTCTCAAACAACAT
TGCAAACAAGGTACTCTTGCAACTCATAGTTAATAATTCTGTTGCAGATTTGAGCAGCTG
ATTGTGAGATGAATTTTCTGGATTATTTACATTATCTTCTAACTGTAAACTTGCAGGCAN
NGAC
STS source: Okitsu 46
STS Forward / Reverse: Reverse read

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): Trovita orange/Kiyomi/Miyagawa-wase
Comment:
Figure legend:
Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Resriction enzyme: NC
Rank as CAPS marker: Others
Comment: PCR_condition: 56C aneal. PCR unstable.
Figure legend:
CVName 1. Nagami, 2. Budha's, 3. Miyagawa, 4. Trovita, 5. Kiyomi, 6. Tachibana, 7. Koji, 8. Kinokuni (8' Hirakishu), 9. Mato pumello, 10. Kunip,
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)

Experiments: CAPS pattern of Citrus collection

Experiments: Segregation pattern

Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: NC
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless

Target on C. clementina (v1.0)

Transcript ID: Ciclev10015870m
Scaffold: scaffold_2
Start: 35334245
End: 35336841

Target on C. unshiu (v1.0)

AGI map info (Shimada et al. 2014)

KmMg map info (Omura et al. 2003)

JtTr map info (Omura et al. 2003)

Km/O41 map info (Nakano et al. 2003)

Rp/Sa map info (Ohta et al. 2011)

CAPS genotyping (Hybrid cv/Citrus collection)

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)