Locus name: Ov0501
CAPS PCR info
PCR Anneal-Temp (℃) / Method: 60Main product (bp): 1600
Comment on PCR:
F-primer: CTCTCCCTACCCCATCCACTC
R-primer: CGCAATGCAACCTCAAGC
EST info
EST Accession number 1: DC894009EST Accession number 2: DC894010
sequence-tagged site (STS seq)
Experiments: Restriction fragment profile
Compared cultivars (Left, (Middle), Right): Trovita orange/Kiyomi/Miyagawa-waseComment: PCR_condition: 62C aneal
Figure legend:
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Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment: PCR_condition: 62C aneal
Figure legend:
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Compared cultivars (Left, (Middle), Right): Frying dragon/Hyuuganatsu
Comment: PCR_condition: 62C aneal
Figure legend:
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Experiments: CAPS pattern of hybrid cultivar
Experiments: CAPS pattern of Citrus collection
Experiments: Segregation pattern
Population name: Kankitsu Chuukanbohon Nou8gou x Siamese AcidlessRestriction enzyme: Msp1
Comment: PCR_condition: 62C aneal
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
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Target on C. clementina (v1.0)
Transcript ID: Ciclev10019938mScaffold: scaffold_3
Start: 48089473
End: 48091232
Target on C. unshiu (v1.0)
Transcript ID: C_unshiu_00026:mRNA_86.1Scaffold: C_unshiu_00026
Start: 712172
End: 713868
Gene function: Nepenthesin II
AGI map info (Shimada et al. 2014)
AGI LG:AGI cM:
KmMg map info (Omura et al. 2003)
KmMg LG:KmMg cM:
JtTr map info (Omura et al. 2003)
JtTr LG:JtTr cM:
Km/O41 map info (Nakano et al. 2003)
Km/O41 LG:Km/O41 cM:
Rp/Sa map info (Ohta et al. 2011)
Rp/Sa LG: RP-05Rp/Sa cM: 61.026
CAPS genotyping (Hybrid cv/Citrus collection)
CAPS genotyping:Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)
Cultivar identification M/T/K:SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)
SNP1:DNA sequence around SNP1:
DNA sequence around SNP2:
DNA sequence around SNP3:
DNA sequence around SNP4: