Citrus CAPS Marker Database

Locus name: Tf0050

CAPS PCR info

PCR Anneal-Temp (℃) / Method: Td
Main product (bp): 800
Comment on PCR:
F-primer: GGCCACCTTGTTGTGAC
R-primer: CTGCTGGTTTTGTGTAGGA

EST info

EST Accession number 1: BQ623221
EST Accession number 2:

sequence-tagged site (STS seq)

STS:
GGCCACCTGTTGTGACAAAATTGGTATCAAGAAAGGACCATGGACTCCAGAAGAAGATAT
CATTTTAGTTTCTTATATTCAAGAGCATGGCCCTGGAAATTGGAGGGCTGTTCCCACTAA
TACAGGTTAATCATATTTTTTTAAATTTTTTCATGATTAATTTGCTTATTTGCATATTAG
TTTGATGAGTAACATACTCTTTAATTTGTTTTCAGGATTGCTTAGATGCAGCAAAAGTTG
CAGGCTTAGATGGACTAATTACCTAAGGCCAGGGATCAAGCGTGGGAATTTCACTGATCA
AGAAGAGAAGATGATAATTCATCTGCAAGCACTTTTGGGCAACAGGTATATTTCCAAAAG
TGCTTTTTCTTTAAGTGCATGCCTTGTTCTTTTATCAGAAGTGATTAGTTGGATGAGCAC
TGTTTGATGGTTGTTGCAGATGGGCTGCTATTGCTTCTTATCTCCCTCAGAGAACTGACA
ATGACATCAAGAACTATTGGAATACTCACTTGAAGAAGAAGGTTAAGAAGCTGCAACTAG
CTGCTGCTGGCTGCTCTGAAGATAATAGCCAATATAGAGATGAGCTAGCTTCAGCTTCTT
CACAGCAAATCTCAAGGGGCCAGTGGGAGAGAAGGCTGCAGACTGATATTCACATGGCTA
AGCAAGCTCTATGTGCGGCCTTGTCACCAGATAAAGCGAGTATTTTGTCTGAATTGAA-G
CCTGCTAA-TGGGTTCATTTCCTACACAAAACCAGCAG
STS source:
STS Forward / Reverse:

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:
Compared cultivars (Left, (Middle), Right): Frying dragon/Hyuuganatsu
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Experiments: CAPS pattern of Citrus collection

Experiments: Segregation pattern

Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Dra1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless

Target on C. clementina (v1.0)

Transcript ID: Ciclev10012152m
Scaffold: scaffold_6
Start: 24874840
End: 24876388

Target on C. unshiu (v1.0)

Transcript ID: C_unshiu_00135:mRNA_12.1
Scaffold: C_unshiu_00135
Start: 84260
End: 85810
Gene function: Myb-related protein 306

AGI map info (Shimada et al. 2014)

AGI LG: AGI-04
AGI cM: 36.106

KmMg map info (Omura et al. 2003)

KmMg LG:
KmMg cM:

JtTr map info (Omura et al. 2003)

JtTr LG:
JtTr cM:

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG:
Km/O41 cM:

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG:
Rp/Sa cM:

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping:

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K:

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1: SI104
DNA sequence around SNP1:
TGCTTTTTCTTTAAGTGCATGCCTT[T/G]TTCTTTTATCAGAAGTGATTAGTTGGATGA
GCACTGTTTGATGGTTGTTGCAGATGGGC
SNP2: SI346
DNA sequence around SNP2:
GGCCACCTGTTGTGACAAAATTGGTATCAAGAAAGG[A/G]CCATGGACTCCAGAAGAAG
ATATCATTTTAGTTTCTTATATTCAAGAGCATGGCCCTGGA
SNP3:
DNA sequence around SNP3:
SNP4:
DNA sequence around SNP4: