Citrus CAPS Marker Database

Locus name: Tf0068

CAPS PCR info

PCR Anneal-Temp (℃) / Method: Td
Main product (bp): 1200
Comment on PCR:
F-primer: GCCGTTTCTGACGAAGACATA
R-primer: TCCATGGCTTGAAGATAGGCT

EST info

EST Accession number 1: CB291749
EST Accession number 2:

sequence-tagged site (STS seq)

STS:

AATACATGAAGTAGTTTCTTGGAGCAGGGGAGGTGTTAGCTTTGTTGTTTGGGACCCTCA
TGCTTTCTCCACTTCTCTTCTTCCTAGACACTTCAAGCACAGTAATTTCTCTAGCTTTGT
CAGGCAGCTTAACACTTACGTAAGTAATGCTATCCTCAATCCCTGTTAGTCTATTAATTA
TTATCAAATGACCCTGGAAATGGCTTCTTCGGCTTTACTCTCAATCAGAATTTCTGAATG
AAACCAAAAGAATGGACTTTGTTTTGTTTTATTTTTATTTATTTATTTTTCCTAAATCAT
CTTCCAATCATTCTTGTTGGGTTTGTAATCTTTTGAAATGCGCAAGCATGTGCAAGCGTG
TTGGACTGTCTAGAAGCCATCCAAGTGGATTCATTTTTATTTAATAAATTATATAGACTG
ATGGTTCTTAGAGCTTCTGGAAACTCACATGAAATCTTTTTATTTCCAATCAGTAGAAAG
TTCGCTAACGCCTTTCCATTTTTTTATTTTTTTTTCTCACTTTTGTTAACTAAATTTACT
GGAGAATCAGTGCTCGGAATTTTTACAATTATTGTCCCTTTTTTCTGACTGTGGGGCCTT
GCTTCATTTCGTTTGCTGGGCACGGAATTTTTTTCCCCCTAATTTTCTGCCGTCATCAAG
TGCATGAATGTTTAATTAAGAATTACTCTGCGAGACGATGTTTAATCAAAGAATAACTCT
TTATGAAATGAATGGATACGGAAGATTTACACGTTCATAAAGTTTCTCTGTTCTGGCAGT
GGATGCTTAGTTAAAATTTTGCTTTTGTTGCAGGGCTTTAGAAAGATCGATCCAGACAGA
TGGGAATTTTCCAATGAAGGGTTTCTGAGGGGAGAGAGGCATCTTTTGAAGAACATCAAG
AGGAGAAAGGCGCCATCGCAGCCTCTGCCTCCGCCACAAGCTCTTGGGCCTTGTGTTGAA
CTGGGCCGATTTGGACTAGACGGGGAATTTGAGCGTCTGATTCGCGACAAACAATTTCTG
ATGATGGAATT

STS source:
STS Forward / Reverse:

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Experiments: CAPS pattern of Citrus collection

Experiments: Segregation pattern

Target on C. clementina (v1.0)

Transcript ID: Ciclev10020724m
Scaffold: scaffold_3
Start: 44141305
End: 44144453

Target on C. unshiu (v1.0)

Transcript ID: C_unshiu_00217:mRNA_25.1
Scaffold: C_unshiu_00217
Start: 201916
End: 205032
Gene function: Heat shock transcription factor A7a1

AGI map info (Shimada et al. 2014)

AGI LG: AGI-05
AGI cM: 54.268

KmMg map info (Omura et al. 2003)

KmMg LG:
KmMg cM:

JtTr map info (Omura et al. 2003)

JtTr LG:
JtTr cM:

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG:
Km/O41 cM:

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG:
Rp/Sa cM:

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping:

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K:

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1: SI106
DNA sequence around SNP1:

GTAATGCTATCCTCAATCCCTGTTAGTCTATTAATTATTATCAAATGACCCTGGAAATGG
[T/C]TTCTTCGGCTTTACTCTCAATCAGAATTTCTGAATGAAACCAAAAGAATGGACTT
TGTTT

SNP2:
DNA sequence around SNP2:

SNP3:
DNA sequence around SNP3:

SNP4:
DNA sequence around SNP4: