Citrus CAPS Marker Database

Locus name: Tf0069

CAPS PCR info

PCR Anneal-Temp (℃) / Method: 64
Main product (bp): 800
Comment on PCR:
F-primer: GCAAACAACCGATGGCCGTAG
R-primer: TCCTTTGAACGAGCCGCTGAT

EST info

EST Accession number 1: CB292181
EST Accession number 2:

sequence-tagged site (STS seq)

STS:
ATGCCGGAGACGCCTCAGCACCGTGGGATCCACCACCGTAGGGCCCACTCCGACACCTCC
TTCCGCTTCGATGACTTCTTACTCTTCGACCCCTCCGACCTCGACCTCTCCGCCCTCGAC
CTCCCCTCGCCCAATCCGACGCCGCCACGTGGCGTCCCCATGCCCCTAGATTCCTCCGAG
GACTCCTCGTCCAACAGCCACGGCCAGAAGCAGCAGAAGCAAAGCCAAAACCCGCCGAAG
CCTAAGCCTATCAACCACCTCCGTAGCCTCTCCGTTGACTCTGATTTCTTCGACAGCTTG
GGTTTGATCTCCCCCGCTTCAGGCGGCGGTGCAGGAAACAGTGCGGCGGGGGCGGGAGGA
GGAGGAGAGAAGAGAAGTTACCATAGGCATAGTAACTCAATGGATGGGTTTGAGGTAGAG
TCTATCTTAGGTGACGGTGTTAAGAAGGCCGTGGATCGTGATCGCCTCGCTGAACTCGCC
CTCATCGATCCCAAACGAGCTAAAAGGTTTGCTTTTTCTTCAACTTTTTCACTATTACGA
TGACATCGTTTGATGATTTTGTATTTATTTATATTTGTTTGCCATCTATGTGAAAGAAAA
TCAAAAAATAGTATTTCATATGTGTTAATATATATCTTTCTCAAGAGTGAAATTAACTTT
TGGGAGTGGGTACAAAGATTGTTCTAATGGGATATGATCTTTTTTGTAGGATTTTAGCTA
ATAGGCAATCAGCGGCTCGTTCAAAG
STS source:
STS Forward / Reverse:
STS:
CCCAAGCTGTCGAAGAAATCAGAGTCAACGGAGAGGCTACGGAGGTGGTTGATAGGCTTA
GGCTTCGGCGGGTTTTGGCTTTGCTTCTGCTGCTTCTGGCCGTGGCTGTTGGACGAGGAG
TCCTCGGAGGAATCTAGGGGCATGGGGACGCCACGTGGCGGCGTCGGATTGGGCGAGGGG
AGGTCGAGGGCGGAGAGGTCGAGGTCTGAGGGGTCGAAGAGTAAGAAGTCATCGAAGCGG
AAGGAGGTGTCGGAGT
STS source:
STS Forward / Reverse:

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:
Compared cultivars (Left, (Middle), Right): Shiamese acidless/RP-94
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Experiments: CAPS pattern of Citrus collection

Experiments: Segregation pattern

Population name: Kankitsu Chuukanbohon Nou8gou x Siamese Acidless
Restriction enzyme: Msp1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Msp1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Sty1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless

Target on C. clementina (v1.0)

Transcript ID: Ciclev10001715m
Scaffold: scaffold_5
Start: 38566192
End: 38571337

Target on C. unshiu (v1.0)

Transcript ID: C_unshiu_00183:mRNA_2.1
Scaffold: C_unshiu_00183
Start: 20134
End: 26910
Gene function: Transcription factor RF2b

AGI map info (Shimada et al. 2014)

AGI LG: AGI-09
AGI cM: 42.789

KmMg map info (Omura et al. 2003)

KmMg LG:
KmMg cM:

JtTr map info (Omura et al. 2003)

JtTr LG:
JtTr cM:

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG:
Km/O41 cM:

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG: RP-09
Rp/Sa cM: 77.126

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping:

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K:

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1: SI199
DNA sequence around SNP1:
CCGTAGGGCCCACTCCGACACCTCCTTCCGCTTCGATGACTTCTTACTCTTCGACCCCTC
[A/C]GACCTCGACCTCTCCGCCCTCGACCTCCCCTCGCCCAATCCGACGCCGCCACGTG
GCGTC
SNP2:
DNA sequence around SNP2:
SNP3:
DNA sequence around SNP3:
SNP4:
DNA sequence around SNP4: