Citrus CAPS Marker Database

Locus name: Tf0071

CAPS PCR info

PCR Anneal-Temp (℃) / Method: Td
Main product (bp): 900
Comment on PCR: Additional band
F-primer: AAGCTGGCCATCCGATCAGAT
R-primer: TTGCAATTGCCTTACGGGTAT

EST info

EST Accession number 1: CB292689
EST Accession number 2:

sequence-tagged site (STS seq)

STS:

AAAAAATTTAAACAAATGATGAGCAGCCAATTTGAACTTGAAAGAATCATCTAATCAGAA
ATTCATAGATCAAACCGTCAACGCAGAATCTGTGGTCTCAGCGCAATGAGCGATAGTGTG
AGCCTGATTGATCCTCCTCCGACGGAGTCCCGCGACGCGGCACGAATTGCGGATGTGAAA
GCGTGGCTGGCGTCACAGTTCGAAGCGTCCGCAAAAGAGGTCCCCGCCTTCGAATACACG
TCTCGGAGCGTTGCGCATTTGTACAACCTCGCAACTCTCTCGCAGGCCAAAACTAAAGCC
GCCAACATCGTCGCCGCCGATTTTCGCCAAAAAGCCGCCGAATATCGATCACAAGGCATC
TTTTTTTTCTCTTTACAATTTTCATTTCTTATTAAAACTGTTATCATCTCCCCCCCCCCC
CCCCCCCCCCCCCCCCCCCACCCACCAAAAAAAAGTTATTTATTTATTTGTTTGATGGTT
TTTGTAGCTGCTAGGATAAGAGAGATATTGGAGAATGTAGGGTTGGCTCAAGAGAGTTTG
CCCTCTAATGTGGTTTCGTCGGCGCAAGTTCTTGCAAATGTAGCTAATTTATTAAATATA
AGAGACACTGAACTTAGTAGGTGGGTTGGGTATCATTTGTTTCACCGTTATTTATCATTT
TTATTTATTTATTGTTGAGTTTTTAAAGCTTAATTGGATGAGGTTTATTCAGTTTCCTTG
TAGCAATGGGCGACATTTCGCTGAGAAAAACTGCCGTGGAGGAGAAGAGAGCTAAAGTAC
AGAAGGAGTCCAAAATTCTT

STS source:
STS Forward / Reverse:

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Experiments: CAPS pattern of Citrus collection

Experiments: Segregation pattern

Target on C. clementina (v1.0)

Transcript ID: Ciclev10022102m
Scaffold: scaffold_3
Start: 26610747
End: 26612959

Target on C. unshiu (v1.0)

Transcript ID: C_unshiu_00167:mRNA_6.1
Scaffold: C_unshiu_00167
Start: 57300
End: 62494
Gene function: HAUS augmin-like complex subunit

AGI map info (Shimada et al. 2014)

AGI LG: AGI-03
AGI cM: 46.274

KmMg map info (Omura et al. 2003)

KmMg LG:
KmMg cM:

JtTr map info (Omura et al. 2003)

JtTr LG:
JtTr cM:

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG:
Km/O41 cM:

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG:
Rp/Sa cM:

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping:

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K:

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1: SI084
DNA sequence around SNP1:

CGCAAAAGAGGTCCCCGCCTTCGAATACACGTCTCGGAGCGTTGCGCATTTGTACAACCT
[C/G]GCAACTCTCTCGCAGGCCAAAACTAAAGCCGCCAACATCGTCGCCGCCGATTTTC
GCCAA

SNP2: SI146
DNA sequence around SNP2:

CAGTTTCCTTGTAGCAATGGGCGACATTTCGCTGAGAAAAACTGCCGTGGAGGAGAAGAG
[A/G]GCTAAAGTACAGAAGGAGTCCAAAATTCTT

SNP3:
DNA sequence around SNP3:

SNP4:
DNA sequence around SNP4: