Citrus CAPS Marker Database

Locus name: Tf0077

CAPS PCR info

PCR Anneal-Temp (℃) / Method: Td
Main product (bp): 1500
Comment on PCR:
F-primer: GAGGAAATATTAGACGACGAA
R-primer: CGCCAATTGTAACCATC

EST info

EST Accession number 1: CB293496
EST Accession number 2:

sequence-tagged site (STS seq)

STS:
GGAGCGAGTCAACTCACTGAGCCACACCAACTGCAACTCACCGAGAATAATAAACACTAT
AAAGGTAGTTTCTTTCTTTCTTTCGCTTTTTTTATTTTTCATTTTTATTTTAGTTTTGAG
AGTGATACGGATGATCCGGCAGGTTTACAAGTGAATTCTGGAGTGGAATCCAATGAAGGA
ACTAAACTGAAGGTGGGAAACTGAAGCTGAGTTGTTTATTTTTGAGTTAACTGCTTAAAT
AGTGATTTGCTCAGTTGAATAGATAGTTTAGTCGGTGCTTGTGGGATTCTTTGTAGCTTA
TACTGCGTTGAGAAATGGTTTCAACTTATTAGTTTCATGTTTTTCCAAGAAACGGAAGAA
TAAAGATTTAAACTTGAAGTGGAGTAAGTACTGGCTGCGGAATTTAATGTTTATTTAGTG
CTCGTTTGAGGTTGGGGTTTAGGGTTTAACTAAACCTCAGATTAGGCTTATGGAAACCAT
ATTTAGGGTTTAACTACTCTCATACCTTGATAGTTTTCTCTTTTTGTTGGCTACACGTTT
CTCCTGTTTAATCATGTTAGAGTATGTGCCTGGTTACTCTATGTACAGGGTTCTTCTGTC
TTGTGCTTTTGTTTTTTTTTGTTTTTGGCATTGATGTTTGTATGTGCTTTCATATTCTTT
TGAATTCAGCACATTTTATTACTCTATTGGTACTATGTATTTTGGAAGTGAGAAAAGAAA
ATGGGCATATGCTAACTAACTAATGCTTTTTTCGGAGAAACCTATTTTTAAATGCGTGTG
AGGCGTGCACCTCTCATCCTATTCTGAGCGAGCTGAATGTTATTATAAATGTTGGCTCTA
AGTCATAACTGCATAAGCCATGCCATGGATTGGTCATGAGGCACACTCATCATCCTCCAC
ATGCCATGTTTAGCATTGATTTAGATGCATCTTCAATAATTTAGGTAAAGTATATATAAA
TGGAAACTCCAACTATGTTTACAAGCCAATCCGGTTGCTTGTGCATTGATTTTTTTCCCG
ATCTTATTATTCTTTTCATCAGCTATTTCCTGTTTCATCCTTATCAGGAGCAGGTTGAGG
TGTCTCACCATGAAGGTTCGGGAAGTATAGCGGTCCAGGCTGCTGAATCTCAGACACAAT
ATCAACTTCAGCCATCCATATGCCCAACATCCTTATCTGAGCTATCACCTACTTCTGTTA
CAGAACCTATATCATCTGCTCCAAGTCCAACTTTAACAGGACAAAACTGTCACTT
GTAAAGGTTGATACTGCATCTGTGCCAGAAGCAAACTTGCAAAACTCTT
STS source:
STS Forward / Reverse:

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:
Compared cultivars (Left, (Middle), Right): Frying dragon/Hyuuganatsu
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Experiments: CAPS pattern of Citrus collection

Rsetriction enzyme: Dra1
Comment: DNA sample: New CCN
Figure legend:
CV (No.1 Top) 101 Tahiti, 100 Rangpurlime, 102 Limonia, 104 LisbonL, 99 SweetLime, 92 Hirado, 87 MGF, 85 Kinukawa, 84 Hassaku, 83 Yamamikan, 78 Natsudaidai, 58 Rokugatu, Tr Trovita, 50 WNV, 44 Tankan, 42 Iyo,
(No.1 Bottom) 39 HgNt, 36 Ujukitsu, 32 Kawabata, 30 Shunkokan, 70 Yuzu, 54 Henka, 53 Ichang lemon, 37 Kunenbo, Mg Miyagawa, 31 Yatsushiro, 27 Kabuchii, 26 Ponkan, 25 Medet. 22. Dancy, 14 Tachibana, 11 Hirakishu (26' Ohta ponkan)
(No.2 Top) 6 Cleopatra, 4 CQ, 2 Fukuremikan, FD Hiryuu, 124 Largeleaf, 125 Small leaf, 141 CTV-S, 136 Rubidoaux,
(No.2 Bottom) FD, Hassaku, Km, F1, RP94, SA, 02, 04, 05, 06, 07, 08, 09, 10, 11, 13
BDZ BenDi Zao, SO Shuto, BP Banpeiyu
Rsetriction enzyme: Rsa1
Comment: DNA sample: New CCN
Figure legend:
CV (No.1 Top) 101 Tahiti, 100 Rangpurlime, 102 Limonia, 104 LisbonL, 99 SweetLime, 92 Hirado, 87 MGF, 85 Kinukawa, 84 Hassaku, 83 Yamamikan, 78 Natsudaidai, 58 Rokugatu, Tr Trovita, 50 WNV, 44 Tankan, 42 Iyo,
(No.1 Bottom) 39 HgNt, 36 Ujukitsu, 32 Kawabata, 30 Shunkokan, 70 Yuzu, 54 Henka, 53 Ichang lemon, 37 Kunenbo, Mg Miyagawa, 31 Yatsushiro, 27 Kabuchii, 26 Ponkan, 25 Medet. 22. Dancy, 14 Tachibana, 11 Hirakishu (26' Ohta ponkan)
(No.2 Top) 6 Cleopatra, 4 CQ, 2 Fukuremikan, FD Hiryuu, 124 Largeleaf, 125 Small leaf, 141 CTV-S, 136 Rubidoaux,
(No.2 Bottom) FD, Hassaku, Km, F1, RP94, SA, 02, 04, 05, 06, 07, 08, 09, 10, 11, 13
BDZ BenDi Zao, SO Shuto, BP Banpeiyu

Experiments: Segregation pattern

Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Rsa1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless

Target on C. clementina (v1.0)

Transcript ID: Ciclev10008135m
Scaffold: scaffold_1
Start: 28433972
End: 28437921

Target on C. unshiu (v1.0)

Transcript ID: C_unshiu_00590:mRNA_15.1
Scaffold: C_unshiu_00590
Start: 86430
End: 90131
Gene function: WRKY

AGI map info (Shimada et al. 2014)

AGI LG: AGI-01
AGI cM: 34.814

KmMg map info (Omura et al. 2003)

KmMg LG:
KmMg cM:

JtTr map info (Omura et al. 2003)

JtTr LG:
JtTr cM:

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG:
Km/O41 cM:

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG:
Rp/Sa cM:

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping:

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K:

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1: SI135
DNA sequence around SNP1:
GGAGCGAGTCAACTCACTGAGCCACACCAACTGCAACTCACCGAGAATAATAAACAC[T/
C]ATAAAGGTAGTTTCTTTCTTTCTTTCGCTTTTTTTATTTTTCATTTTTATTTTAGTTT
TG
SNP2:
DNA sequence around SNP2:
SNP3:
DNA sequence around SNP3:
SNP4:
DNA sequence around SNP4: