Locus name: Tf0150
CAPS PCR info
PCR Anneal-Temp (℃) / Method: TdMain product (bp): 500
Comment on PCR:
F-primer: ACAGAAGAGGCCACAATCT
R-primer: TTTCTTCAGCTAAAGCGTCAC
EST info
EST Accession number 1: DC886590EST Accession number 2: DC886591
sequence-tagged site (STS seq)
STS:| ATGAGAATTCACAAGCAATCCAACACGAAAGGAAAAGGACCCTGTACTTACAAGCAGTTG AACATCCACAACTTTGCAAAAGATTTGTGGTTGGAGATCAAAGTTGGGAACTTCAATAGG TGGAAAAGCAGAGGATGAGGCAACTTGTTCTAAGTGACCCTGAGGGAAGTAAACAACTAC ATTTCCTTTCTTGGGCAGTGAAGTGAGAGGACCAGCACAAGCATGCCAAAGCTCAAAATA AATTGAAGAATATAAAGAAGAAGAAGAAGAAGAATTTGCAGAACATGAAGAAGAAGAAGA TGAAGAGTTCAAGTAACAGACACAACCACTGTTGTTCTTCCCACAATCTCCATTGCAAAA TGCAATCTTTTCCCCCTCAGTAGTTGCTGCATGGTTCAGATCAAATTCCATGAACACCTC AGCCGA |
STS Forward / Reverse: Reverse read
STS:
| GCGAGCATTAAATTCATCACACACCAAACTAATCATTTGAAGCAATAAATAAAGATTCTT GAACTAGCTAGAGATGTTTGTTTAATTCGGATGACATTAATTACCTGGAAAAGAGAGTGC CACAGTCGCATCTATACTCTCGAGTGCCGCAAATCTTACTATGAGCTTTCCAATCTGATT GAACTGCATATTTCTTTGAACATTTCTCACACTTCCATTTCTTTTCCCCCATGTTTTCTG CTGAAGTGCTTCTTTATTCCAGTGAGGTCACCTAGGGCTCTTGATGGATCATGATGCACG |
STS Forward / Reverse:
Experiments: Restriction fragment profile
Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)Comment:
Figure legend:
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Compared cultivars (Left, (Middle), Right): Frying dragon/Hyuuganatsu
Comment:
Figure legend:
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Experiments: CAPS pattern of hybrid cultivar
Resriction enzyme: Hinf1Rank as CAPS marker: Available marker
Comment: DNA sample: New CCN
Figure legend: DNA sample: Lane 9 (Mato) and 25 (Mineola) not analyzed.
CVName 1. Nagami, 2. Budha's, 3. Miyagawa, 4. Trovita, 5. Kiyomi, 6. Tachibana, 7. Koji, 8. Kinokuni (8' Hirakishu), 9. Mato pumello, 10. Kunip,
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)
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Experiments: CAPS pattern of Citrus collection
Rsetriction enzyme: Hinf1Comment: DNA sample: New CCN
Figure legend:
CV (No.1 Top) 101 Tahiti, 100 Rangpurlime, 102 Limonia, 104 LisbonL, 99 SweetLime, 92 Hirado, 87 MGF, 85 Kinukawa, 84 Hassaku, 83 Yamamikan, 78 Natsudaidai, 58 Rokugatu, Tr Trovita, 50 WNV, 44 Tankan, 42 Iyo,
(No.1 Bottom) 39 HgNt, 36 Ujukitsu, 32 Kawabata, 30 Shunkokan, 70 Yuzu, 54 Henka, 53 Ichang lemon, 37 Kunenbo, Mg Miyagawa, 31 Yatsushiro, 27 Kabuchii, 26 Ponkan, 25 Medet. 22. Dancy, 14 Tachibana, 11 Hirakishu (26' Ohta ponkan)
(No.1 Bottom) 39 HgNt, 36 Ujukitsu, 32 Kawabata, 30 Shunkokan, 70 Yuzu, 54 Henka, 53 Ichang lemon, 37 Kunenbo, Mg Miyagawa, 31 Yatsushiro, 27 Kabuchii, 26 Ponkan, 25 Medet. 22. Dancy, 14 Tachibana, 11 Hirakishu (26' Ohta ponkan)
(No.2 Top) 6 Cleopatra, 4 CQ, 2 Fukuremikan, FD Hiryuu, 124 Largeleaf, 125 Small leaf, 141 CTV-S, 136 Rubidoaux,
(No.2 Bottom) FD, Hassaku, Km, F1, RP94, SA, 02, 04, 05, 06, 07, 08, 09, 10, 11, 13
(No.2 Bottom) FD, Hassaku, Km, F1, RP94, SA, 02, 04, 05, 06, 07, 08, 09, 10, 11, 13
BDZ BenDi Zao, SO Shuto, BP Banpeiyu
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Experiments: Segregation pattern
Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)Restriction enzyme: Hinf1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
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Target on C. clementina (v1.0)
Transcript ID: Ciclev10028292mScaffold: scaffold_8
Start: 22191056
End: 22193554
Target on C. unshiu (v1.0)
Transcript ID: C_unshiu_01354:mRNA_7.2Scaffold: C_unshiu_01354
Start: 51348
End: 53833
Gene function: Zinc finger protein JACKDAW
AGI map info (Shimada et al. 2014)
AGI LG: AGI-08AGI cM: 25.22
KmMg map info (Omura et al. 2003)
KmMg LG:KmMg cM:
JtTr map info (Omura et al. 2003)
JtTr LG:JtTr cM:
Km/O41 map info (Nakano et al. 2003)
Km/O41 LG:Km/O41 cM:
Rp/Sa map info (Ohta et al. 2011)
Rp/Sa LG:Rp/Sa cM:
CAPS genotyping (Hybrid cv/Citrus collection)
CAPS genotyping: Hb, CcCultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)
Cultivar identification M/T/K: M,KSNP typing by Illumina GoldenGate assay (Fujii et al. 2013)
SNP1: SI262DNA sequence around SNP1:
| TCGAGTGCCGCAAATCTTACTATGAGCTTTCCAATCTGATTGAACTGCATATTTCTTTGA [A/G]CATTTCTCACACTTCCATTTCTTTTCCCCCATGTTTTCTGCTGAAGTGCTTCTTT ATTCC |
DNA sequence around SNP2:
DNA sequence around SNP3:
DNA sequence around SNP4: