Locus name: Tf0154
CAPS PCR info
PCR Anneal-Temp (℃) / Method: 64
Main product (bp): 1400
Comment on PCR:
F-primer: CCGGTGGTAAGGGTAGTGTGA
R-primer: CAAACGTCTCGCCTGC
EST info
EST Accession number 1: DC889992
EST Accession number 2:
sequence-tagged site (STS seq)
STS:
TCTCTTCAAGGTGCTCTGAAGCCTCTTGTCATCGGTGGTTGTTGTCTTATGAACAGCCTT CTTCTTTCTGTGATTTAAAGCAAGTGTCAAAAAAGAATTTACCATATGATAGACACAACC TAAAATTCAAATATAGTTCATAATGGAAAGGAAAATTTTATTGAAATGCCAACCTACAAA ATAAACTTCAATTAACAGAGGAAATAATGCACAAAGCTGAAGTATCTACATCTCTTACAC CATTCACATAGACAGCAACAAATTAAAATACAGAACACAGAATAGGCTAGTATCTACAAA TAAGCTCAAGTATCTATTCCTCTCACTCTTACTCTCTTGTTTTAGTTTACTACCAGTCCC AGATGACAAAAAGTTGTAAAGAAGAGATATATACAAGAAAAACTCTAAGTCTCAGAGAAA ACAGCCAACAATATAAATAATTAAGTCATGACACAAACAAGC |
STS source:
STS Forward / Reverse: Combined sequence of both reads
Experiments: Restriction fragment profile
Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:
Experiments: CAPS pattern of hybrid cultivar
Resriction enzyme: Hae3
Rank as CAPS marker: Available marker
Comment: DNA sample: New CCN
Figure legend: DNA sample: Lane 9 (Mato) not analyzed.
CVName 1. Nagami, 2. Budha's, 3. Miyagawa, 4. Trovita, 5. Kiyomi, 6. Tachibana, 7. Koji, 8. Kinokuni (8' Hirakishu), 9. Mato pumello, 10. Kunip,
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)
Experiments: CAPS pattern of Citrus collection
Rsetriction enzyme: Hae3
Comment: DNA sample: New CCN
Figure legend:
CV (No.1 Top) 101 Tahiti, 100 Rangpurlime, 102 Limonia, 104 LisbonL, 99 SweetLime, 92 Hirado, 87 MGF, 85 Kinukawa, 84 Hassaku, 83 Yamamikan, 78 Natsudaidai, 58 Rokugatu, Tr Trovita, 50 WNV, 44 Tankan, 42 Iyo,
(No.1 Bottom) 39 HgNt, 36 Ujukitsu, 32 Kawabata, 30 Shunkokan, 70 Yuzu, 54 Henka, 53 Ichang lemon, 37 Kunenbo, Mg Miyagawa, 31 Yatsushiro, 27 Kabuchii, 26 Ponkan, 25 Medet. 22. Dancy, 14 Tachibana, 11 Hirakishu (26' Ohta ponkan)
(No.2 Top) 6 Cleopatra, 4 CQ, 2 Fukuremikan, FD Hiryuu, 124 Largeleaf, 125 Small leaf, 141 CTV-S, 136 Rubidoaux,
(No.2 Bottom) FD, Hassaku, Km, F1, RP94, SA, 02, 04, 05, 06, 07, 08, 09, 10, 11, 13
BDZ BenDi Zao, SO Shuto, BP Banpeiyu
Experiments: Segregation pattern
Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Hae3
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Target on C. clementina (v1.0)
Transcript ID: Ciclev10022594m
Scaffold: scaffold_3
Start: 16957427
End: 16959735
Target on C. unshiu (v1.0)
Transcript ID: C_unshiu_01562:mRNA_2.1
Scaffold: C_unshiu_01562
Start: 12059
End: 15864
Gene function: Nascent polypeptide-associated complex subunit beta
AGI map info (Shimada et al. 2014)
AGI LG:
AGI cM:
KmMg map info (Omura et al. 2003)
KmMg LG:
KmMg cM:
JtTr map info (Omura et al. 2003)
JtTr LG:
JtTr cM:
Km/O41 map info (Nakano et al. 2003)
Km/O41 LG:
Km/O41 cM:
Rp/Sa map info (Ohta et al. 2011)
Rp/Sa LG:
Rp/Sa cM:
CAPS genotyping (Hybrid cv/Citrus collection)
CAPS genotyping: Hb, Cc
Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)
Cultivar identification M/T/K:
SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)
SNP1:
DNA sequence around SNP1:
SNP2:
DNA sequence around SNP2:
SNP3:
DNA sequence around SNP3:
SNP4:
DNA sequence around SNP4: