Citrus CAPS Marker Database

Locus name: Tf0219

CAPS PCR info

PCR Anneal-Temp (℃) / Method: Td
Main product (bp): 600
Comment on PCR:
F-primer: ACTTGCAGCAGTCAATCCA
R-primer: GGGAAGCTACTGTCGATTAAC

EST info

EST Accession number 1: CD576023
EST Accession number 2:

sequence-tagged site (STS seq)

STS:
ACGAGTCTTTACAGACATAACTACAACAGTCAAGTGCATTTAGCTCAGGCCATGACAATC
ATTTTGATTGTCGGAAGTGTAAATTTGAGCAGGTAAAATAGGGTGGTCCTAGTCTGACCC
ACTCCCCTGTACAGGTTTTAAAGGCAAACAGTCACACTCTGGTGTAGGAATAATCCAATG
AGCTAGATTACGATTTCAAAGATTTCTGACTTCCTGTTCCATGAAAATAATAAAACTAGG
CCTATTTAAATTAAAATTACAACAACTACACAACAAATAAATGATGCAGTGGCTAATAGC
TATTCAGGAAGGTTAAACCAAGGAGCAGCTCTTTATGGGAACAATGTACTAGGCTATAGA
TCAAACCAACCCACAACATAACCAGTCTACACCTTG
STS source:
STS Forward / Reverse:

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:
Compared cultivars (Left, (Middle), Right): Frying dragon/Hyuuganatsu
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Resriction enzyme: EcoR5
Rank as CAPS marker: no homozygous cultivar with minor allele
Comment: DNA sample: New CCN
Figure legend: DNA sample: Lane 25 (Mineola) not analyzed.
CVName 1. Nagami, 2. Budha's, 3. Miyagawa, 4. Trovita, 5. Kiyomi, 6. Tachibana, 7. Koji, 8. Kinokuni (8' Hirakishu), 9. Mato pumello, 10. Kunip,
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)

Experiments: CAPS pattern of Citrus collection

Rsetriction enzyme: EcoR5
Comment: DNA sample: New CCN
Figure legend:
CV (No.1 Top) 101 Tahiti, 100 Rangpurlime, 102 Limonia, 104 LisbonL, 99 SweetLime, 92 Hirado, 87 MGF, 85 Kinukawa, 84 Hassaku, 83 Yamamikan, 78 Natsudaidai, 58 Rokugatu, Tr Trovita, 50 WNV, 44 Tankan, 42 Iyo,
(No.1 Bottom) 39 HgNt, 36 Ujukitsu, 32 Kawabata, 30 Shunkokan, 70 Yuzu, 54 Henka, 53 Ichang lemon, 37 Kunenbo, Mg Miyagawa, 31 Yatsushiro, 27 Kabuchii, 26 Ponkan, 25 Medet. 22. Dancy, 14 Tachibana, 11 Hirakishu (26' Ohta ponkan)
(No.2 Top) 6 Cleopatra, 4 CQ, 2 Fukuremikan, FD Hiryuu, 124 Largeleaf, 125 Small leaf, 141 CTV-S, 136 Rubidoaux,
(No.2 Bottom) FD, Hassaku, Km, F1, RP94, SA, 02, 04, 05, 06, 07, 08, 09, 10, 11, 13
BDZ BenDi Zao, SO Shuto, BP Banpeiyu

Experiments: Segregation pattern

Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: EcoR5
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Sty1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless

Target on C. clementina (v1.0)

Transcript ID: Ciclev10005087m
Scaffold: scaffold_9
Start: 25662049
End: 25666791

Target on C. unshiu (v1.0)

Transcript ID: C_unshiu_00115:mRNA_31.1
Scaffold: C_unshiu_00115
Start: 248109
End: 252610
Gene function: Transcription factor bHLH60

AGI map info (Shimada et al. 2014)

AGI LG: AGI-03
AGI cM: 49.25

KmMg map info (Omura et al. 2003)

KmMg LG:
KmMg cM:

JtTr map info (Omura et al. 2003)

JtTr LG:
JtTr cM:

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG:
Km/O41 cM:

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG:
Rp/Sa cM:

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping: Hb, Cc

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K:

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1: SI076
DNA sequence around SNP1:
ACGAGTCTTTACAGACATAACTACAACA[A/G]TCAAGTGCATTTAGCTCAGGCCATGAC
AATCATTTTGATTGTCGGAAGTGTAAATTTGAG
SNP2: SI267
DNA sequence around SNP2:
TTGATTGTCGGAAGTGTAAATTTGAGCAGGTAAAATAGGGTGGTCCTAGTCTGACCCACT
[T/C]CCCTGTACAGGTTTTAAAGGCAAAC
SNP3:
DNA sequence around SNP3:
SNP4:
DNA sequence around SNP4: