Locus name: Tf0326
CAPS PCR info
PCR Anneal-Temp (℃) / Method: 62
Main product (bp): 1200
Comment on PCR:
F-primer: TTGACGCCACTAAGTA
R-primer: AGCATTTGGGTATCATATCTA
EST info
EST Accession number 1: CK938083
EST Accession number 2:
sequence-tagged site (STS seq)
STS:
GTAATGCTTTAAAGTACTATGAGGTTTATAATTTTAATGAATAAGCATATATTTCATGTC CAGTGATTGTGACGAAGAGAAAAATATGTATTAGCTTTTGATTCTATGATAGCCCAGCTG TATGGGTAACTTCATTAAAATCTAATAXACATTACTCAAGCTACCCTTTAGTAATTTTGT TTGAAGCTGTAAAATTGTACTTTTTTAGGCGGTGGATTATGGAGATTGTTATGTTCTCAT GGAAGAATGCATGATTTTAATCTAATTGAGAGTCGAGGTTGTTTAGTGAAG |
STS source:
STS Forward / Reverse:
Experiments: Restriction fragment profile
Experiments: CAPS pattern of hybrid cultivar
Resriction enzyme: Hha1
Rank as CAPS marker: Available marker
Comment: DNA sample: New CCN
Figure legend: DNA sample: Lane 9 (Mato) not analyzed.
CVName 1. Nagami, 2. Budha's, 3. Miyagawa, 4. Trovita, 5. Kiyomi, 6. Tachibana, 7. Koji, 8. Kinokuni (8' Hirakishu), 9. Mato pumello, 10. Kunip,
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)
Experiments: CAPS pattern of Citrus collection
Rsetriction enzyme: Hha1
Comment: DNA sample: New CCN
Figure legend:
CV (No.1 Top) 101 Tahiti, 100 Rangpurlime, 102 Limonia, 104 LisbonL, 99 SweetLime, 92 Hirado, 87 MGF, 85 Kinukawa, 84 Hassaku, 83 Yamamikan, 78 Natsudaidai, 58 Rokugatu, Tr Trovita, 50 WNV, 44 Tankan, 42 Iyo,
(No.1 Bottom) 39 HgNt, 36 Ujukitsu, 32 Kawabata, 30 Shunkokan, 70 Yuzu, 54 Henka, 53 Ichang lemon, 37 Kunenbo, Mg Miyagawa, 31 Yatsushiro, 27 Kabuchii, 26 Ponkan, 25 Medet. 22. Dancy, 14 Tachibana, 11 Hirakishu (26' Ohta ponkan)
(No.2 Top) 6 Cleopatra, 4 CQ, 2 Fukuremikan, FD Hiryuu, 124 Largeleaf, 125 Small leaf, 141 CTV-S, 136 Rubidoaux,
(No.2 Bottom) FD, Hassaku, Km, F1, RP94, SA, 02, 04, 05, 06, 07, 08, 09, 10, 11, 13
BDZ BenDi Zao, SO Shuto, BP Banpeiyu
Experiments: Segregation pattern
Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Hha1
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: NC
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
Target on C. clementina (v1.0)
Transcript ID: Ciclev10024695m
Scaffold: scaffold_7
Start: 2501520
End: 2509737
Target on C. unshiu (v1.0)
Transcript ID: C_unshiu_02417:mRNA_1.1
Scaffold: C_unshiu_02417
Start: 3692
End: 11684
Gene function: Histone-lysine N-methyltransferase SUVR5
AGI map info (Shimada et al. 2014)
AGI LG: AGI-02
AGI cM: 69.928
KmMg map info (Omura et al. 2003)
KmMg LG:
KmMg cM:
JtTr map info (Omura et al. 2003)
JtTr LG:
JtTr cM:
Km/O41 map info (Nakano et al. 2003)
Km/O41 LG:
Km/O41 cM:
Rp/Sa map info (Ohta et al. 2011)
Rp/Sa LG:
Rp/Sa cM:
CAPS genotyping (Hybrid cv/Citrus collection)
CAPS genotyping: Hb, Cc
Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)
Cultivar identification M/T/K: M,K
SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)
SNP1: SI085
DNA sequence around SNP1:
CTAATAXACATTACTCAAGCTACCCTTTAGTAATTTTGTT[T/G]GAAGCTGTAAAATTG TACTTTTTTAGGCGGTGGATTATGGAGATTGTTATGTTCTCATGG |
SNP2:
DNA sequence around SNP2:
SNP3:
DNA sequence around SNP3:
SNP4:
DNA sequence around SNP4: