Locus name: Tf0353
CAPS PCR info
PCR Anneal-Temp (℃) / Method: 64Main product (bp): 800
Comment on PCR:
F-primer: ATGTACTTCCCTACCCGTAAT
R-primer: GAGGGTAAAGACTCGGAAGC
EST info
EST Accession number 1: CN186263EST Accession number 2:
sequence-tagged site (STS seq)
STS:| CATAGCGCTGCTGTAATGTTCGCTCAAGCTCCTTTTGCTTCTGAACATCCTCCCACAGAC CATTTATTCGGCTCGATGCTGCTAATTGTTCTTGCTTCTGCAAAGCCAGGAAGCACTCCA GCTCTGTTCCAGCTGTTTCCATCTGCTTGACAGTTGAGTGAATTTGAGCCCGCAGGTTTT CAGCTCGTTTCTGTACATTTAAACGAGAATCACTTAGCAATGTAAAACTCTGAATTTAAG CAATAGCAACAAACAAATCAGACAACTAATTATTGTCTTAAATTGCTAAGTGATTCAGCA TAATAGTTTGTTGCTATTTGCATCTCAAACACACTAACTTGTAAAAGAAAGTGTGTTGAG AAATCTAGTAGAATCAATCATGATCAAATTAGTAGAAGTAATAACACGGGGAAATAATCA GCAAGCATACCATAGCATACCTCATAACCTTGTGTGTAAACTTTGACCGTTTTCTCAAGT TGTAAAGCCTTCTCCT |
STS Forward / Reverse:
Experiments: Restriction fragment profile
Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)Comment:
Figure legend:
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Experiments: CAPS pattern of hybrid cultivar
Resriction enzyme: Nde2Rank as CAPS marker: Disagree by Marco analysis
Comment: DNA sample: New CCN
Figure legend: DNA sample: Lane 9 (Mato) not analyzed.
CVName 1. Nagami, 2. Budha's, 3. Miyagawa, 4. Trovita, 5. Kiyomi, 6. Tachibana, 7. Koji, 8. Kinokuni (8' Hirakishu), 9. Mato pumello, 10. Kunip,
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)
11. King, 12. Ponkan (12' Ohta ponkan), 13. Dancy, 14. Willowleaf, 15. Clementine, 16. C-haploid, 17. Encore, 18. Amaka, 19. Youkou, 20. Shiranuhi,
21. Harumi, 22. Nishinokaori, 23. Duncan GF, 24. Mineola, 25. Seminol, 26. Seihou, 27. Akemi, 28. Tsunokaori, 29. Ariake, 30. Nankou,
31. Mihocore, 32. Hareyaka, 34. Harehime, 35. Hayaka, 36. Southern Red, 37. Amakusa, 38. Setoka, *. Flying Dragon, SY(Southern Yellow), 40(MK, Mukakukishu)
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Experiments: CAPS pattern of Citrus collection
Rsetriction enzyme: Nde2Comment: DNA sample: New CCN
Figure legend:
CV (No.1 Top) 101 Tahiti, 100 Rangpurlime, 102 Limonia, 104 LisbonL, 99 SweetLime, 92 Hirado, 87 MGF, 85 Kinukawa, 84 Hassaku, 83 Yamamikan, 78 Natsudaidai, 58 Rokugatu, Tr Trovita, 50 WNV, 44 Tankan, 42 Iyo,
(No.1 Bottom) 39 HgNt, 36 Ujukitsu, 32 Kawabata, 30 Shunkokan, 70 Yuzu, 54 Henka, 53 Ichang lemon, 37 Kunenbo, Mg Miyagawa, 31 Yatsushiro, 27 Kabuchii, 26 Ponkan, 25 Medet. 22. Dancy, 14 Tachibana, 11 Hirakishu (26' Ohta ponkan)
(No.1 Bottom) 39 HgNt, 36 Ujukitsu, 32 Kawabata, 30 Shunkokan, 70 Yuzu, 54 Henka, 53 Ichang lemon, 37 Kunenbo, Mg Miyagawa, 31 Yatsushiro, 27 Kabuchii, 26 Ponkan, 25 Medet. 22. Dancy, 14 Tachibana, 11 Hirakishu (26' Ohta ponkan)
(No.2 Top) 6 Cleopatra, 4 CQ, 2 Fukuremikan, FD Hiryuu, 124 Largeleaf, 125 Small leaf, 141 CTV-S, 136 Rubidoaux,
(No.2 Bottom) FD, Hassaku, Km, F1, RP94, SA, 02, 04, 05, 06, 07, 08, 09, 10, 11, 13
(No.2 Bottom) FD, Hassaku, Km, F1, RP94, SA, 02, 04, 05, 06, 07, 08, 09, 10, 11, 13
BDZ BenDi Zao, SO Shuto, BP Banpeiyu
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Experiments: Segregation pattern
Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)Restriction enzyme: Nde2
Comment:
Figure legend:
A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless
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Target on C. clementina (v1.0)
Transcript ID: Ciclev10018691mScaffold: scaffold_3
Start: 34696661
End: 34702240
Target on C. unshiu (v1.0)
Transcript ID: C_unshiu_00669:mRNA_17.2Scaffold: C_unshiu_00669
Start: 126539
End: 133312
Gene function: Cell division cycle 5-like protein
AGI map info (Shimada et al. 2014)
AGI LG: AGI-08AGI cM: 53.069
KmMg map info (Omura et al. 2003)
KmMg LG:KmMg cM:
JtTr map info (Omura et al. 2003)
JtTr LG:JtTr cM:
Km/O41 map info (Nakano et al. 2003)
Km/O41 LG:Km/O41 cM:
Rp/Sa map info (Ohta et al. 2011)
Rp/Sa LG:Rp/Sa cM:
CAPS genotyping (Hybrid cv/Citrus collection)
CAPS genotyping: HbCultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)
Cultivar identification M/T/K:SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)
SNP1: SI087DNA sequence around SNP1:
| TTAAATTGCTAAGTGATTCAGCATAATAGTTTGTTGCTATTTGCATCTCAAACACACTAA [A/C]TTGTAAAAGAAAGTGTGTTGAGAAATCTAGTAGAATCAATCATGA |
DNA sequence around SNP2:
DNA sequence around SNP3:
DNA sequence around SNP4: