Citrus CAPS Marker Database

Locus name: Wy0015

CAPS PCR info

PCR Anneal-Temp (℃) / Method: 56
Main product (bp): 1100
Comment on PCR:
F-primer: ATTGATATTGCCAGATTGCTT
R-primer: CTTCTTCCCCAAACTTCATT

EST info

EST Accession number 1: DC894276
EST Accession number 2: DC894277

sequence-tagged site (STS seq)

STS:

ATTGGTACACTGTAAATTACCGCGAAGCTTACGGGCTTTTCGCGTGTAACGGAATTTTAT
TTAACCACGAATCGCCTAGACGCGGCGAAAATTTTGTTACTAGAAAAATTACGCGTGCGG
TTGGGAGGATCAAGATAGGATTGCAGAGTAAATTGTTTTTGGGTAATTTGCAAGCGTCGA
GGGATTGGGGATTTGCGGGAGACTACGTGGAGGCAATGTGGATGATGTTGCAGCAGGAGA
AGCCGGATGATTATGTGGTGGCGACGGAGGAGTCGCATACAGTGGAGGAGTTTTTGGAGG
TCGCGTTTGGGTATGTGGGGTTGAATTGGAAGGATCATGTGGTGATCGATAAGAGGTATT
T

STS source:
STS Forward / Reverse:

Experiments: Restriction fragment profile

Compared cultivars (Left, (Middle), Right): Trovita orange/Kiyomi/Miyagawa-wase
Comment:
Figure legend:

Compared cultivars (Left, (Middle), Right): A255(Okitsu46gou)/G434(Kankitsu Chuukanbohon Nou5)
Comment:
Figure legend:

Experiments: CAPS pattern of hybrid cultivar

Experiments: CAPS pattern of Citrus collection

Rsetriction enzyme: Hinf1
Comment:
Figure legend:

CV (No.1 Top) 101 Tahiti, 100 Rangpurlime, 102 Limonia, 104 LisbonL, 99 SweetLime, 92 Hirado, 87 MGF, 85 Kinukawa, 84 Hassaku, 83 Yamamikan, 78 Natsudaidai, 58 Rokugatu, Tr Trovita, 50 WNV, 44 Tankan, 42 Iyo,
(No.1 Bottom) 39 HgNt, 36 Ujukitsu, 32 Kawabata, 30 Shunkokan, 70 Yuzu, 54 Henka, 53 Ichang lemon, 37 Kunenbo, Mg Miyagawa, 31 Yatsushiro, 27 Kabuchii, 26 Ponkan, 25 Medet. 22. Dancy, 14 Tachibana, 11 Hirakishu (26' Ohta ponkan)

(No.2 Top) 6 Cleopatra, 4 CQ, 2 Fukuremikan, FD Hiryuu, 124 Largeleaf, 125 Small leaf, 141 CTV-S, 136 Rubidoaux,
(No.2 Bottom) FD, Hassaku, Km, F1, RP94, SA, 02, 04, 05, 06, 07, 08, 09, 10, 11, 13

BDZ BenDi Zao, SO Shuto, BP Banpeiyu

Rsetriction enzyme: Msp1
Comment:
Figure legend:

(No.2 Top) 6 Cleopatra, 4 CQ, 2 Fukuremikan, FD Hiryuu, 124 Largeleaf, 125 Small leaf, 141 CTV-S, 136 Rubidoaux,
(No.2 Bottom) FD, Hassaku, Km, F1, RP94, SA, 02, 04, 05, 06, 07, 08, 09, 10, 11, 13

BDZ BenDi Zao, SO Shuto, BP Banpeiyu

Experiments: Segregation pattern

Population name: A255 (Okitsu46gou) x G434 (Kankitsu Chuukanbohon Nou5gou)
Restriction enzyme: Hinf1
Comment:
Figure legend:

A: A255 (Okitu46gou), G: G434 (Kankitsu Chuukanbohon Nou5gou),
K or Km: Kiyomi, M or Mg: Miyagawa-wase, MK: Mukaku kisyu,
LE: Lee, O: Okitsu41gou, RP or RP94: Kankitsu Chukanbohon Nou6gou,
Sacd or SA: Siamese Acidless, SW: Sweet spring, T or Tr: Trovita orange,
X: meaningless

Population name: Jutaro unshu x Trovita orange
Restriction enzyme: Hinf1
Comment:
Figure legend:

Population name: Kiyomi x Miyagawa-wase
Restriction enzyme: Hinf1
Comment:
Figure legend:

Target on C. clementina (v1.0)

Transcript ID: Ciclev10028465m
Scaffold: scaffold_8
Start: 13101257
End: 13103161

Target on C. unshiu (v1.0)

Transcript ID: C_unshiu_02878:mRNA_1.1
Scaffold: C_unshiu_02878
Start: 9199
End: 10715
Gene function: UDP-glycosyltransferase 73C5

AGI map info (Shimada et al. 2014)

AGI LG: AGI-08
AGI cM: 56.724

KmMg map info (Omura et al. 2003)

KmMg LG:
KmMg cM:

JtTr map info (Omura et al. 2003)

JtTr LG:
JtTr cM:

Km/O41 map info (Nakano et al. 2003)

Km/O41 LG:
Km/O41 cM:

Rp/Sa map info (Ohta et al. 2011)

Rp/Sa LG:
Rp/Sa cM:

CAPS genotyping (Hybrid cv/Citrus collection)

CAPS genotyping:

Cultivar identification (M: Cultivar identification manual / T: Ninomiya et al. 2015/ K: Nonaka et al. 2017)

Cultivar identification M/T/K:

SNP typing by Illumina GoldenGate assay (Fujii et al. 2013)

SNP1: SI041
DNA sequence around SNP1:

TGTAACGGAATTTTATTTAACCACGAATCGCCTAGACG[T/C]GGCGAAAATTTTGTTAC
TAGAAAAATTACGCGTGCGGTTGGGAGGATCAAGATAGGATTG

SNP2:
DNA sequence around SNP2:

SNP3:
DNA sequence around SNP3:

SNP4:
DNA sequence around SNP4: